Vent DNA polymerase

Vent polymerase is a thermostable archean DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis.[1]

References

Vigneault F, Drouin R (2005). "Optimal conditions and specific characteristics of Vent exo- DNA polymerase in ligation-mediated polymerase chain reaction protocols". Biochem Cell Biol. 83 (2): 147–65. doi:10.1139/o04-134. PMID 15864324.

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Polymerase chain reaction techniques
Procedure	Initialization cycle Denaturation Annealing elongation Final elongation Final hold
Polymerase	Klenow T4 and T7 Taq Tth Pfu Vent Pwo Tli Tfu
Optimization ＆ variants	Quantitative polymerase chain reaction (qPCR) Reverse transcription polymerase chain reaction (RT-PCR) Inverse polymerase chain reaction (Inverse PCR) Nested polymerase chain reaction (Nested PCR) Touchdown polymerase chain reaction Hot start PCR Overlap extension polymerase chain reaction (OE-PCR) Multiplex polymerase chain reaction (Multiplex-PCR) Multiplex ligation-dependent probe amplification (MLPA) co-amplification at lower denaturation temperature-PCR (COLD-PCR)
History ＆ people	Kary Mullis Kjell Kleppe Alice Chien