Retinol binding protein 4

Retinol binding protein 4, also known as RBP4, is a transporter protein[5] for retinol (vitamin A alcohol). RBP4 has a molecular weight of approximately 21 kDa and is encoded by the RBP4 gene in humans.[6][7] It is mainly, though not exclusively, synthesized in the liver and circulates in the bloodstream bound to retinol in a complex with transthyretin. RBP4 has been a drug target for ophthalmology research due to its role in vision.[8] RBP4 may also be involved in metabolic diseases as suggested by recent studies.

RBP4
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesRBP4, MCOPCB10, RDCCAS, retinol binding protein 4
External IDsOMIM: 180250 MGI: 97879 HomoloGene: 4908 GeneCards: RBP4
Gene location (Human)
Chr.Chromosome 10 (human)[1]
Band10q23.33Start93,591,687 bp[1]
End93,601,744 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

5950

19662

Ensembl

ENSG00000138207

ENSMUSG00000024990

UniProt

P02753

Q00724

RefSeq (mRNA)

NM_006744
NM_001323517
NM_001323518

NM_001159487
NM_011255

RefSeq (protein)

NP_001310446
NP_001310447
NP_006735

NP_001152959
NP_035385

Location (UCSC)Chr 10: 93.59 – 93.6 MbChr 19: 38.12 – 38.13 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Function

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.[7]

Structure
Two molecules of RBP4 (in yellow and red) bound to retinol (in orange) complexed with four molecules of TTR (in purple and blue)

RBP4 is a single polypeptide chain with a hydrophobic pocket where retinol binds. The RBP4-retinol complex then binds transthyretin in circulation to prevent renal filtration of RBP4.[9]

In serum, TTR and RBP4 bind in a 1 to 1 stoichiometry (two molecules of TTR combine with two molecules of RBP4 to form a complex with a total molecular weight of approximately 80,000 Daltons).[10]

Clinical significance

Retinol-binding protein 4 has been a drug target for eye diseases as RBP4 is the sole carrier for retinol, which is an essential nutrient for the visual cycle. Animal studies using RBP4-antagonists showed that lowering RBP4 can lead to reduction in the accumulation of lipofuscin that leads to vision loss in eye diseases like Stargardt's disease and macular degeneration.[8][11]An animal study using ABCA4 knockout mouse proved that reduction in serum RBP4 level could inhibit lipofuscin without inhibiting the visual cycle.[ref] One clinical study in age-related macular degeneration (AMD) was conducted using Fenretinide. The study showed trends in reducing lesion growth rate in AMD and rate of conversion from early stage AMD (dry AMD) to late stage AMD (wet AMD) without serious side effects.

RBP4 has recently been described as an adipokine that contributes to insulin resistance and diabetes in the AG4KO mouse model.[12] In addition to the liver, RBP4 is also secreted by adipocytes of the fat tissue in a smaller portion and acts as a signal to surrounding cells, when there is a decrease in plasma glucose concentration.[13] It is suspected that an elevated level of RBP4 attracts macrophages to the fat tissue, causes local inflammation, and leads to insulin resistance.[14][15]

Mutations in the RBP4 gene have recently been linked to a form of autosomal dominant microphthalmia, anophthalmia, and coloboma (MAC) disease.[16] A unique feature of this disease is the maternal inheritance effect, when a fetus inherits a mutated copy of the RBP4 gene from its mother, but not from its father. The physiologic basis lies in pregnancy whereby the mutated gene product, retinol binding protein (RBP), has negative effects in transferring vitamin A from maternal liver storage sites to the placenta, and then again on the fetal circulation side when delivering vitamin A from the placenta to developing fetal tissues, most notably the developing eye. This 'double whammy' effect does not exist when the mutant RBP4 gene is inherited from the father. The above mechanism is separate from previously known types of maternal inheritance effects such as genomic imprinting, mitochondrial inheritance, or maternal oocyte mRNA transfer. The authors of the above study cite the potential of vitamin A supplementation in pregnant females who are known to carry an RBP4 mutation with retinyl ester which utilizes an RBP-independent pathway to deliver retinoids from the maternal intestines directly to the placenta and ultimately is uptaken by the fetus. The key would be to supplement during the first several months of life when the eye begins to develop, as supplementing later in pregnancy would be too late to avoid any potential MAC disease.

See also

References
  1. GRCh38: Ensembl release 89: ENSG00000138207 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000024990 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Rask L, Anundi H, Fohlman J, Peterson PA (1987). "The complete amino acid sequence of human serum retinol-binding protein". Upsala Journal of Medical Sciences. 92 (2): 115–46. doi:10.3109/03009738709178685. PMID 2444024.
  6. Rocchi M, Covone A, Romeo G, Faraonio R, Colantuoni V (March 1989). "Regional mapping of RBP4 to 10q23----q24 and RBP1 to 3q21----q22 in man". Somatic Cell and Molecular Genetics. 15 (2): 185–90. doi:10.1007/BF01535081. PMID 2928844. S2CID 37896530.
  7. "Entrez Gene: RBP4 retinol binding protein 4, plasma".
  8. Cioffi CL, Dobri N, Freeman EE, Conlon MP, Chen P, Stafford DG, Schwarz DM, Golden KC, Zhu L, Kitchen DB, Barnes KD, Racz B, Qin Q, Michelotti E, Cywin CL, Martin WH, Pearson PG, Johnson G, Petrukhin K (September 2014). "Design, synthesis, and evaluation of nonretinoid retinol binding protein 4 antagonists for the potential treatment of atrophic age-related macular degeneration and Stargardt disease". Journal of Medicinal Chemistry. 57 (18): 7731–57. doi:10.1021/jm5010013. PMC 4174998. PMID 25210858.
  9. Kanai M, Raz A, Goodman DS (September 1968). "Retinol-binding protein: the transport protein for vitamin A in human plasma". The Journal of Clinical Investigation. 47 (9): 2025–44. doi:10.1172/jci105889. PMC 297364. PMID 5675424.
  10. Naylor HM, Newcomer ME (March 1999). "The structure of human retinol-binding protein (RBP) with its carrier protein transthyretin reveals an interaction with the carboxy terminus of RBP". Biochemistry. 38 (9): 2647–53. doi:10.1021/bi982291i. PMID 10052934.
  11. Radu RA, Han Y, Bui TV, Nusinowitz S, Bok D, Lichter J, Widder K, Travis GH, Mata NL (December 2005). "Reductions in serum vitamin A arrest accumulation of toxic retinal fluorophores: a potential therapy for treatment of lipofuscin-based retinal diseases". Investigative Ophthalmology & Visual Science. 46 (12): 4393–401. doi:10.1167/iovs.05-0820. PMID 16303925.
  12. Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB (July 2005). "Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes". Nature. 436 (7049): 356–62. doi:10.1038/nature03711. PMID 16034410. S2CID 4343960.
  13. Herman MA, Kahn BB (July 2006). "Glucose transport and sensing in the maintenance of glucose homeostasis and metabolic harmony". The Journal of Clinical Investigation. 116 (7): 1767–75. doi:10.1172/JCI29027. PMC 1483149. PMID 16823474.
  14. Moraes-Vieira PM, Yore MM, Dwyer PM, Syed I, Aryal P, Kahn BB (March 2014). "RBP4 activates antigen-presenting cells, leading to adipose tissue inflammation and systemic insulin resistance". Cell Metabolism. 19 (3): 512–26. doi:10.1016/j.cmet.2014.01.018. PMC 4078000. PMID 24606904.
  15. Galic S, Oakhill JS, Steinberg GR (March 2010). "Adipose tissue as an endocrine organ". Molecular and Cellular Endocrinology. 316 (2): 129–39. doi:10.1016/j.mce.2009.08.018. PMID 19723556. S2CID 7385199.
  16. Chou CM, Nelson C, Tarlé SA, Pribila JT, Bardakjian T, Woods S, Schneider A, Glaser T (April 2015). "Biochemical Basis for Dominant Inheritance, Variable Penetrance, and Maternal Effects in RBP4 Congenital Eye Disease". Cell. 161 (3): 634–646. doi:10.1016/j.cell.2015.03.006. PMC 4409664. PMID 25910211.

Further reading


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