RFC4

Replication factor C subunit 4 is a protein that in humans is encoded by the RFC4 gene.[5][6]

RFC4
Identifiers
Aliases	RFC4, A1, RFC37, replication factor C subunit 4
External IDs	OMIM: 102577 MGI: 2146571 HomoloGene: 6288 GeneCards: RFC4
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3q27.3 Start 186,789,880 bp[1] End 186,807,058 bp[1]
Gene location (Mouse) Chr. Chromosome 16 (mouse)[2] Band 16|16 B1 Start 23,113,943 bp[2] End 23,127,737 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • nucleotide binding • DNA binding • GO:0043142 single-stranded DNA helicase activity • GO:0001948 protein binding • enzyme binding • ATP binding • DNA clamp loader activity Cellular component • DNA replication factor C complex • Ctf18 RFC-like complex • cell nucleus • nucleoplasm • Elg1 RFC-like complex Biological process • nucleotide-excision repair, DNA gap filling • error-free translesion synthesis • DNA strand elongation involved in DNA replication • error-prone translesion synthesis • DNA replication • positive regulation of DNA-directed DNA polymerase activity • translesion synthesis • transcription-coupled nucleotide-excision repair • nucleotide-excision repair, DNA incision • DNA damage response, detection of DNA damage • nucleotide-excision repair, DNA incision, 5'-to lesion • DNA repair • telomere maintenance via semi-conservative replication • DNA-dependent DNA replication • regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5984	106344
Ensembl	ENSG00000163918	ENSMUSG00000022881
UniProt	P35249	Q99J62
RefSeq (mRNA)	NM_181573 NM_002916	NM_145480
RefSeq (protein)	NP_002907 NP_853551	NP_663455
Location (UCSC)	Chr 3: 186.79 – 186.81 Mb	Chr 16: 23.11 – 23.13 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.[6]

Interactions

RFC4 has been shown to interact with:

• BRD4,[7]
• CHTF18,[8]
• PCNA,[9][10]
• RFC2,[9][11]
• RFC3,[12][13] and
• RFC5.[9][11][12][14]

References

1. GRCh38: Ensembl release 89: ENSG00000163918 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022881 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K (Jan 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics. 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID 7774928.
6. "Entrez Gene: RFC4 replication factor C (activator 1) 4, 37kDa".
7. Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Molecular and Cellular Biology. 22 (18): 6509–20. doi:10.1128/mcb.22.18.6509-6520.2002. PMC 135621. PMID 12192049.
8. Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proceedings of the National Academy of Sciences of the United States of America. 100 (18): 10237–42. doi:10.1073/pnas.1434308100. PMC 193545. PMID 12930902.
9. Cai J, Gibbs E, Uhlmann F, Phillips B, Yao N, O'Donnell M, Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". The Journal of Biological Chemistry. 272 (30): 18974–81. doi:10.1074/jbc.272.30.18974. PMID 9228079.
10. Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
11. Cai J, Yao N, Gibbs E, Finkelstein J, Phillips B, O'Donnell M, Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proceedings of the National Academy of Sciences of the United States of America. 95 (20): 11607–12. doi:10.1073/pnas.95.20.11607. PMC 21688. PMID 9751713.
12. Ellison V, Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". The Journal of Biological Chemistry. 273 (10): 5979–87. doi:10.1074/jbc.273.10.5979. PMID 9488738.
13. Uhlmann F, Cai J, Flores-Rozas H, Dean FB, Finkelstein J, O'Donnell M, Hurwitz J (Jun 1996). "In vitro reconstitution of human replication factor C from its five subunits". Proceedings of the National Academy of Sciences of the United States of America. 93 (13): 6521–6. doi:10.1073/pnas.93.13.6521. PMC 39056. PMID 8692848.
14. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

Further reading

• Chen M, Pan ZQ, Hurwitz J (Jun 1992). "Studies of the cloned 37-kDa subunit of activator 1 (replication factor C) of HeLa cells". Proceedings of the National Academy of Sciences of the United States of America. 89 (12): 5211–5. doi:10.1073/pnas.89.12.5211. PMC 49261. PMID 1351677.
• Uhlmann F, Cai J, Flores-Rozas H, Dean FB, Finkelstein J, O'Donnell M, Hurwitz J (Jun 1996). "In vitro reconstitution of human replication factor C from its five subunits". Proceedings of the National Academy of Sciences of the United States of America. 93 (13): 6521–6. doi:10.1073/pnas.93.13.6521. PMC 39056. PMID 8692848.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (Apr 1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Molecular and Cellular Biology. 17 (4): 1817–23. doi:10.1128/MCB.17.4.1817. PMC 232028. PMID 9121429.
• Cai J, Gibbs E, Uhlmann F, Phillips B, Yao N, O'Donnell M, Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". The Journal of Biological Chemistry. 272 (30): 18974–81. doi:10.1074/jbc.272.30.18974. PMID 9228079.
• Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (Dec 1997). "Identification of DNA replication and cell cycle proteins that interact with PCNA". Nucleic Acids Research. 25 (24): 5041–6. doi:10.1093/nar/25.24.5041. PMC 147130. PMID 9396813.
• Ellison V, Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". The Journal of Biological Chemistry. 273 (10): 5979–87. doi:10.1074/jbc.273.10.5979. PMID 9488738.
• Zhang G, Gibbs E, Kelman Z, O'Donnell M, Hurwitz J (Mar 1999). "Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen". Proceedings of the National Academy of Sciences of the United States of America. 96 (5): 1869–74. doi:10.1073/pnas.96.5.1869. PMC 26703. PMID 10051561.
• Uchiumi F, Watanabe M, Tanuma S (May 1999). "Characterization of telomere-binding activity of replication factor C large subunit p140". Biochemical and Biophysical Research Communications. 258 (2): 482–9. doi:10.1006/bbrc.1999.0589. PMID 10329413.
• van der Kuip H, Carius B, Haque SJ, Williams BR, Huber C, Fischer T (Apr 1999). "The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G1 phase cell cycle regulatory proteins". Journal of Molecular Medicine. 77 (4): 386–92. doi:10.1007/s001090050365. PMID 10353443. S2CID 22183443.
• Chaib H, Cockrell EK, Rubin MA, Macoska JA (2001). "Profiling and verification of gene expression patterns in normal and malignant human prostate tissues by cDNA microarray analysis". Neoplasia. 3 (1): 43–52. doi:10.1038/sj.neo.7900126. PMC 1505021. PMID 11326315.
• Naiki T, Kondo T, Nakada D, Matsumoto K, Sugimoto K (Sep 2001). "Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway". Molecular and Cellular Biology. 21 (17): 5838–45. doi:10.1128/MCB.21.17.5838-5845.2001. PMC 87303. PMID 11486023.
• Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (Sep 2001). "Purification and characterization of human DNA damage checkpoint Rad complexes". Proceedings of the National Academy of Sciences of the United States of America. 98 (20): 11236–41. doi:10.1073/pnas.201373498. PMC 58713. PMID 11572977.
• Griffith JD, Lindsey-Boltz LA, Sancar A (May 2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". The Journal of Biological Chemistry. 277 (18): 15233–6. doi:10.1074/jbc.C200129200. PMID 11907025.
• Anderson LA, Perkins ND (Aug 2002). "The large subunit of replication factor C interacts with the histone deacetylase, HDAC1". The Journal of Biological Chemistry. 277 (33): 29550–4. doi:10.1074/jbc.M200513200. PMID 12045192.
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.
• Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Molecular and Cellular Biology. 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. PMC 135621. PMID 12192049.
• Anderson LA, Perkins ND (Jan 2003). "Regulation of RelA (p65) function by the large subunit of replication factor C". Molecular and Cellular Biology. 23 (2): 721–32. doi:10.1128/MCB.23.2.721-732.2003. PMC 151544. PMID 12509469.
• Nagata T, Takahashi Y, Ishii Y, Asai S, Sugahara M, Nishida Y, Murata A, Chin M, Schichino H, Koshinaga T, Fukuzawa M, Mugishima H (Jun 2003). "Profiling of genes differentially expressed between fetal liver and postnatal liver using high-density oligonucleotide DNA array". International Journal of Molecular Medicine. 11 (6): 713–21. doi:10.3892/ijmm.11.6.713. PMID 12736711.