List of restriction enzyme cutting sites: S

This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 
Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

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Restriction enzymes

S
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
SacI [7] Streptomyces achromogenes 5' GAGCTC
3' CTCGAG 		5' ---GAGCT   C--- 3'
3' ---C   TCGAG--- 5'
SacII Streptomyces achromogenes 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5' 		Cfr42I
SacNI Streptomyces achromogenes N-J-H 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
SalI [7] Streptomyces albus 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
SalPI Streptomyces albus 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI
SanDI Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 		5' ---GG   GWCCC--- 3'
3' ---CCCWG   GG--- 5'
SapI Saccharopolyspora sp. 5' GCTCTTC
3' CGAGAAG 		5' ---GCTCTTCN   NNN--- 3'
3' ---CGAGAAGNNNN   --- 5'
SarI Streptomyces aureomonopodiales 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI
SatI Staphylococcus arlettae RFL832 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
SauI Streptomyces aureofaciens IKA 18/4 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
Sau96I Staphylococcus aureus PS96 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI
Sau3239I Streptomyces aureofaciens 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
Sau3AI 2REU Staphylococcus aureus 3A 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I
SauBMKI Streptomyces aureofaciens BM-K 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SauHPI Streptomyces aureofaciens R8/26 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SauLPI Streptomyces aureofaciens B-96 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SauLPII Streptomyces aureofaciens B-96 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
SauMI Staphylococcus aureus M 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI
SauNI Streptomyces aureofaciens NMU 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SauSI Streptomyces aureofaciens 16 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SbfI Streptomyces sp. BF-61 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
Sbi68I Streptomyces bikiniensis JAM68 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
Sbo13I Shigella boydii 13 5' TCGCGA
3' AGCGCT 		5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
SbvI Streptococcus bovis II/1 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
ScaI [7] Streptomyces caespitosus 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
DpaI, Eco255I, RflFII, ZrmI
SceIII Saccharomyces cerevisiae 5' GCCGGC
3' CGGCCG 		5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
SchI Staphylococcus cohnii Lki 19-320 5' GAGTC
3' CTCAG 		5' ---GAGTCN4N   --- 3'
3' ---CTCAGN4N   --- 5'
SchZI Streptomyces chusanensis ZS-2 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SciI Streptoverticillium cinnamoneum 5' CTCGAG
3' GAGCTC 		5' ---CTC   GAG--- 3'
3' ---GAG   CTC--- 5' 		BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI
SciNI Spiroplasma citri ASP2 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI
ScrFI Streptococcus cremoris F 5' CCNGG
3' GGNCC 		5' ---CC   NGG--- 3'
3' ---GGN   CC--- 5'
SdaI 2IXS Streptomyces diastaticus Ng7-324 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
SdiI Streptomyces diastaticus 5' GGCCN5GGCC
3' CCGGN5CCGG 		5' ---GGCCNNNN   NGGCC--- 3'
3' ---CCGGN   NNNNCCGG--- 5'
SduI Streptococcus durans RFL3 5' GDGCHC
3' CHCGDG 		5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5' 		AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII
SecI Synechocystis sp. 6701 5' CCNNGG
3' GGNNCC 		5' ---C   CNNGG--- 3'
3' ---GGNNC   C--- 5'
SelI Synechococcus elongatus 5' CGCG
3' GCGC 		5' ---   CGCG--- 3'
3' ---GCGC   --- 5' 		AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI
SenPT16I Salmonella enteritidis PT16 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII
SenPT14bI Salmonella enteritidis PT14b 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SepI Staphylococcus epidermidis 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I
SexAI Streptomyces exfoliatus 5' ACCWGGT
3' TGGWCCA 		5' ---A   CCWGGT--- 3'
3' ---TGGWCC   A--- 5'
SexBI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SexCI Streptomyces exfoliatus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SfaI Streptococcus faecalis zymogenes 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
SfaNI Streptococcus faecalis ND547 5' GCATC
3' CGTAG 		5' ---GCATCN4N   NNNN--- 3'
3' ---CGTAGN4NNNNN   --- 5'
SfcI Streptococcus faecium 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
SfeI Streptococcus faecalis 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
SfiI 2F03 Streptomyces fimbriatus 5' GGCCN5GGCC
3' CCGGN5CCGG 		5' ---GGCCNNNN   NGGCC--- 3'
3' ---CCGGN   NNNNCCGG--- 5'
SflI Streptoverticillium flavopersicum 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI
SfoI Serratia fonticola 5' GGCGCC
3' CCGCGG 		5' ---GGC   GCC--- 3'
3' ---CCG   CGG--- 5'
Sfr274I Streptomyces fradiae 274 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Sfr303I Streptomyces fradiae 303 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SfuI Streptomyces fulvissimus 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI
SgfI Streptomyces griseoruber 5' GCGATCGC
3' CGCTAGCG 		5' ---GCGAT   CGC--- 3'
3' ---CGC   TAGCG--- 5'
AsiSI,  RgaI, SfaAI 
SgrAI 3DPG 3N7B 3N78 3MGY 3MG6 Streptomyces griseus 5' CRCCGGYG
3' GYGGCCRC 		5' ---CR   CCGGYG--- 3'
3' ---GYGGCC   RC--- 5'
SgrBI Streptomyces griseus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SimI Staphylococcus intermedius 6H 5' GGGTC
3' CCCAG 		5' ---GG   GTC--- 3'
3' ---CCCAG   --- 5'
SinI Salmonella infantis 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
SlaI Streptomyces lavendulae 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
SleI Synechococcus leopoliensis 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
Slu1777I Streptomyces lusitanus 1777 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SmaI Serratia marcescens Sb 5' CCCGGG
3' GGGCCC 		5' ---CCC   GGG--- 3'
3' ---GGG   CCC--- 5' 		AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI
SmiI Streptococcus milleri S 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
SmiMI Sphingobacterium mizutae M 5' CAYN4RTG
3' GTRN4YAC 		5' ---CAYNN   NNRTG--- 3'
3' ---GTRNN   NNYAC--- 5'
SmlI Stenotrophomonas maltophilia 5' CTYRAG
3' GARYTC 		5' ---C   TYRAG--- 3'
3' ---GARYT   C--- 5'
SmuI Sphingobacterium multivorum RFL21 5' CCCGC
3' GGGCG 		5' ---CCCGCNNNN   NN--- 3'
3' ---GGGCGNNNNNN   --- 5'
SmuEI Streptococcus mutans E 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI
SnaBI Sphaerotilus natans 5' TACGTA
3' ATGCAT 		5' ---TAC   GTA--- 3'
3' ---ATG   CAT--- 5'
SniI Streptomyces niveus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI
SnoI Streptomyces novocastria 5' GTGCAC
3' CACGTG 		5' ---G   TGCAC--- 3'
3' ---CACGT   G--- 5' 		Alw44I, ApaLI, VneI
SolI Streptoverticillium olivoverticillatum 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI
Sol10179I Streptomyces olivochromogenes ST10179 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI
SpaHI Synechococus parietina 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
SpeI Sphaerotilus natans 5' ACTAGT
3' TGATCA 		5' ---A   CTAGT--- 3'
3' ---TGATC   A--- 5' 		AhII, AclNI, BcuI
SphI [7] Streptomyces phaeochromogenes 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
SplI Spirulina platensis 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
SpmI Sphingobacterium multivorum RFL21 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I
SpoI Salmonella potsdam 5' TCGCGA
3' AGCGCT 		5' ---TCG   CGA--- 3'
3' ---AGC   GCT--- 5'
SpuI Streptomyces pulveraceus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
SrfI Streptomyces sp. 5' GCCCGGGC
3' CGGGCCCG 		5' ---GCCC   GGGC--- 3'
3' ---CGGG   CCCG--- 5'
SrlI Selenomonas ruminantium lactilytica 84 5' GCCGGC
3' CGGCCG 		5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
Srl5DI Selenomonas ruminantium 5 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI
Srl32DII Selenomonas ruminantium 32 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
Srl55DI Selenomonas ruminantium lactilytica 55 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
Srl56DI Selenomonas ruminantium 56 5' CTRYAG
3' GAYRTC 		5' ---C   TRYAG--- 3'
3' ---GAYRT   C--- 5'
SruI Selenomonas ruminantium 18D 5' TTTAAA
3' AAATTT 		5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5' 		AhaIII, DraI, PauAII
Sru4DI Selenomonas ruminantium 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, AsnI, BpoAI, PshBI, VspI
Sru30DI Selenomonas ruminantium 30 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI
SsbI Streptomyces scabies 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
SscL1I Staphylococcus sp. L1 5' GANTC
3' CTNAG 		5' ---G   ANTC--- 3'
3' ---CTNA   G--- 5'
Sse9I Sporosarcina sp. 9 5' AATT
3' TTAA 		5' ---   AATT--- 3'
3' ---TTAA   --- 5'
Sse232I Streptomyces sp. RH232 5' CGCCGGCG
3' GCGGCCGC 		5' ---CG   CCGGCG--- 3'
3' ---GCGGCC   GC--- 5'
Sse1825I Streptomyces sp. 5' GGGWCCC
3' CCCWGGG 		5' ---GG   GWCCC--- 3'
3' ---CCCWG   GG--- 5'
Sse8387I Streptomyces sp. 8387 5' CCTGCAGG
3' GGACGTCC 		5' ---CCTGCA   GG--- 3'
3' ---GG   ACGTCC--- 5'
Sse8647I Streptomyces sp. 5' AGGWCCT
3' TCCWGGA 		5' ---AG   GWCCT--- 3'
3' ---TCCWG   GA--- 5'
SseAI Streptomyces sp. 5' GGCGCC
3' CCGCGG 		5' ---GG   CGCC--- 3'
3' ---CCGC   GG--- 5'
SseBI Streptomyces sp. 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI
SshAI Salmonella shikmonah 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
SsiI Staphylococcus sciuri RFL1 5' CCGC
3' GGCG 		5' ---C   CGC--- 3'
3' ---GGC   G--- 5' 		AciI
SsiAI Staphylococcus sp. A 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
SsiBI Staphylococcus sp. B 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
SslI Streptococcus salivarius thermophilus 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI
SsoI Shigella sonnei 47 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
SsoII Shigella sonnei 47 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
SspI Sphaerotilus sp. 5' AATATT
3' TTATAA 		5' ---AAT   ATT--- 3'
3' ---TTA   TAA--- 5'
Ssp1I Streptomyces sp. RFL1 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI
Ssp4800I Streptomyces sp. BMTU 4800 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, SspBI
Ssp5230I Streptomyces sp. 5230 5' GACGTC
3' CTGCAG 		5' ---GACGT   C--- 3'
3' ---C   TGCAG--- 5' 		AatII, ZraI
Ssp27144I Synechococcus sp. 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI
SspAI Synechococcus sp. 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI
SspBI Streptomyces sp. 5' TGTACA
3' ACATGT 		5' ---T   GTACA--- 3'
3' ---ACATG   T--- 5'
AauI,  BsmRI,  Bsp1407I,
BsrGI, BstAUI, Ssp4800I
SspCI Streptomyces sp. 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
SspD5I Staphylococcus sp. D5 5' GGTGA
3' CCACT 		5' ---GGTGAN7N   --- 3'
3' ---CCACTN7N   --- 5' 		AsuHPI, HphI
SspD5II Staphylococcus sp. D5 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I
SspRFI Synechococcus sp. RF-1 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI
SsrI Staphylococcus saprophyticus B6 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
SstI Streptomyces stanford 5' GAGCTC
3' CTCGAG 		5' ---GAGCT   C--- 3'
3' ---C   TCGAG--- 5'
SstII Streptomyces stanford 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Sst12I Streptomyces sp. ST-12 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI
SteI Streptomyces tendae 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI
SthI Salmonella thompson YY356 5' GGTACC
3' CCATGG 		5' ---G   GTACC--- 3'
3' ---CCATG   G--- 5' 		Acc65I, AhaB8I, Asp718I, KpnI,
Sth117I Streptococcus thermophilus ST117 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI
Sth132I Streptococcus thermophilus 5' CCCG
3' GGGC 		5' ---CCCGNNNN   NNNN--- 3'
3' ---GGGCNNNNNNNN   --- 5'
Sth134I Streptococcus thermophilus 134 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Sth368I Streptococcus thermophilus CNRZ368 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI
StrI Streptomyces thermodiastaticus 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI
StsI Streptococcus sanguis 54 5' GGATG
3' CCTAC 		5' ---GGATGN8NN   NNNN--- 3'
3' ---CCTACN8NNNNNN   --- 5'
StuI [8] Streptomyces tubercidicus 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI
StyI Salmonella typhi 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
StyD4I Salmonella typhi D4 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
SuaI Sulfolobus acidocaldarius 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
SuiI Sulfolobus islandicus REN2HI 5' GCWGC
3' CGWCG 		5' ---G   CWGC--- 3'
3' --- CGWC   G--- 5' 		AceI, ApeKI, Taq52I, TseI
SunI Synechococcus uniformis 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
SurI Sporosarcina ureae 2 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI
SviI Streptomyces violochromogenes 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I
SwaI Staphylococcus warneri 5' ATTTAAAT
3' TAAATTTA 		5' ---ATTT   AAAT--- 3'
3' ---TAAA   TTTA--- 5'
This list is incomplete; you can help by adding missing items with reliable sources.

Notes
  1. Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
  7. Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.
  8. Shimotsu H, Takahashi H, Saito H (November 1980). "A new site-specific endonuclease StuI from Streptomyces tubercidicus". Gene. 11 (3–4): 219–25. doi:10.1016/0378-1119(80)90062-1. PMID 6260571.
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