List of restriction enzyme cutting sites: E–F

This article contains a list of the most studied restriction enzymes whose names start with E to F inclusive. It contains approximately 110 enzymes.

Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 

Whole list navigation

Restriction enzymes

E
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
EacI Eubacterium acidaminophilum 5' GGATC
3' CCTAG 		5' ---GGATCNNNN   N--- 3'
3' ---CCTAGNNNNN   --- 5'
AclWI, AlwI, BinI,  BpuFI,  BspPI,
BstH9I,  Bst31TI, Bth617I, Ral8I 
EaeI Enterobacter aerogenes 5' YGGCCR
3' RCCGGY 		5' ---Y   GGCCR--- 3'
3' ---RCCGG   Y--- 5'
Eae46I Enterobacter aerogenes 46 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
EaeAI Enterobacter aerogenes 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EclRI, PaeBI, PspAI, PspALI, XcyI, XmaI, XmaCI
EagI Enterobacter agglomerans 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIII
EagBI Enterobacter agglomerans CBNU45 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorII
EagMI Enterobacter agglomerans M3 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI
Eam1104I Enterobacter amnigenus RFL1104 5' CTCTTC
3' GAGAAG 		5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
Eam1105I Enterobacter amnigenus RFL1105 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5'
AhdI, AspEI,  BmeRI,  BspOVI,
DriI, EclHKI, NruGI
EarI Enterobacter aerogenes 5' CTCTTC
3' GAGAAG 		5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
EcaI Enterobacter cloacae 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I
EciI Escherichia coli 5' GGCGGA
3' CCGCCT 		5' ---GGCGGAN8NNN   --- 3'
3' ---CCGCCTN8N   NN--- 5'
Eci125I Escherichia coli VKM-125 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I
EclI Enterobacter cloacae 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
Ecl136II Enterobacter cloacae RFL136 5' GAGCTC
3' CTCGAG 		5' ---GAG   CTC--- 3'
3' ---CTC   GAG--- 5'
EclHKI Enterobacter cloacae 5' GACN5GTC
3' CTGN5CAG 		5' ---GACNNN   NNGTC--- 3'
3' ---CTGNN   NNNCAG--- 5'
AhdI, AspEI,  BmeRI,  BspOVI,
DriI, Eam1105I, NruGI
EclRI Enterobacter cloacae R 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI, XcyI, XmaI, XmaCI
EclXI Enterobacter cloacae 590 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIII
Ecl18kI 2FQZ Enterobacter cloacae 18k 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Ecl37kI Enterobacter cloacae 37k 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenI
Ecl2zI Enterobacter cloacae 2z 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenI
Eco24I Escherichia coli RFL24 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
Eco31I Escherichia coli RFL31 5' GGTCTC
3' CCAGAG 		5' ---GGTCTCN   NNNN--- 3'
3' ---CCAGAGNNNNN   --- 5'
Eco32I Escherichia coli RFL32 5' GATATC
3' CTATAG 		5' ---GAT   ATC--- 3'
3' ---CTA   TAG--- 5'
Eco47I Escherichia coli RFL47 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI
Eco47III Escherichia coli RFL47 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, AitI, Aor51H, FunI
Eco52I Escherichia coli RFL52 5' CGGCCG
3' GCCGGC 		5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5' 		AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIII
Eco56I Escherichia coli RFL56 5' GCCGGC
3' CGGCCG 		5' ---G   CCGGC--- 3'
3' ---CGGCC   G--- 5'
Eco57I Escherichia coli RFL57 5' CTGAAG
3' GACTTC 		5' ---CTGAAGN12NNNN   --- 3'
3' ---GACTTCN12NN   NN--- 5' 		AcuI, BspKT5I
Eco64I Escherichia coli RFL64 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAI
Eco72I Escherichia coli RFL72 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCI
Eco78I Escherichia coli RFL78 5' GGCGCC
3' CCGCGG 		5' ---GGC   GCC--- 3'
3' ---CCG   CGG--- 5'
Eco81I Escherichia coli RFL81 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Eco88I Escherichia coli RFL88 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAI
Eco91I Escherichia coli RFL91 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAII
Eco105I Escherichia coli RFL105 5' TACGTA
3' ATGCAT 		5' ---TAC   GTA--- 3'
3' ---ATG   CAT--- 5'
Eco130I Escherichia coli RFL130 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
Eco147I Escherichia coli RFL147 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI
Eco255I Escherichia coli RFL255 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
DpaI, RflFII, ScaI, ZrmI
Eco1831I Escherichia coli RFL1831 5' CCSGG
3' GGSCC 		5' ---   CCSGG--- 3'
3' ---GGSCC   --- 5'
AhaI, AseII, AsuC2I, BcnI,
 BpuMI,  CauII, EcoHI, HgiS22I
EcoA4I Escherichia coli A4 5' GGTCTC
3' CCAGAG 		5' ---GGTCTCN   NNNN--- 3'
3' ---CCAGAGNNNNN   --- 5'
EcoHI Escherichia coli HI 5' CCSGG
3' GGSCC 		5' ---   CCSGG--- 3'
3' ---GGSCC   --- 5' 		AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22I, Kpn49kII, NciI
EcoHK31I Escherichia coli HK31 5' YGGCCR
3' RCCGGY 		5' ---Y   GGCCR--- 3'
3' ---RCCGG   Y--- 5'
EcoICRI Escherichia coli 2bT 5' GAGCTC
3' CTCGAG 		5' ---GAG   CTC--- 3'
3' ---CTC   GAG--- 5'
Eco75KI Escherichia coli BKM 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
Eco57MI Escherichia coli RFL57M 5' CTGRAG
3' GACYTC 		5' ---CTGRAGN12NNNN   --- 3'
3' ---GACYTCN12NN   NN--- 5'
EcoNI Escherichia coli 5' CCTN5AGG
3' GGAN5TCC 		5' ---CCTNN   NNNAGG--- 3'
3' ---GGANNN   NNTCC--- 5'
EcoO44I Escherichia coli O44 Hiromi 5' GGTCTC
3' CCAGAG 		5' ---GGTCTCN   NNNN--- 3'
3' ---CCAGAGNNNNN   --- 5'
EcoO65I Escherichia coli K11a 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, Bse64I, BstEII, BstPI, BstT9I, EcaI, Eco91I, EcoO128I
EcoO109I 1WTD Escherichia coli H709c 5' RGGNCCY
3' YCCNGGR 		5' ---RG   GNCCY--- 3'
3' ---YCCNG   GR--- 5'
EcoO128I Escherichia coli O128Ly3 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AspAI, Bse64I, BstEII, BstT9I, EcaI, EcoO65I, NspSAII, PspEI
EcoP15I Escherichia coli P15 5' CAGCAG
3' GTCGTC 		5' ---CAGCAGN25NN   --- 3'
3' ---GTCGTCN25   NN--- 5'
EcoR124II 2y3t, 2w74 Search Escherichia coli 5' 5'-GAAN7RTCG-3'
5'  
 
EcoRI 1QC9, Search EcoR1 structures Escherichia coli RY13 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
EcoRII 1NA6 Escherichia coli R245 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI, ZanI
EcoRV 1RVE Search EcoRV Escherichia coli J62 pLG74 5' GATATC
3' CTATAG 		5' ---GAT   ATC--- 3'
3' ---CTA   TAG--- 5'
EcoT14I Escherichia coli TB14 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
EcoT22I Escherichia coli TB22 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5II
EcoT38I Escherichia coli TH38 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
EcoVIII Escherichia coli E1585-68 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
Eco13kI Escherichia coli 13k 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Eco21kI Escherichia coli 21k 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Eco27kI Escherichia coli 27 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAI
Eco29kI Escherichia coli 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Eco53kI Escherichia coli 53k 5' GAGCTC
3' CTCGAG 		5' ---GAG   CTC--- 3'
3' ---CTC   GAG--- 5'
Eco137kI Escherichia coli 137k 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
EgeI Enterobacter gergoviae NA 5' GGCGCC
3' CCGCGG 		5' ---GGC   GCC--- 3'
3' ---CCG   CGG--- 5'
EheI Erwinia herbicola 9/5 5' GGCGCC
3' CCGCGG 		5' ---GGC   GCC--- 3'
3' ---CCG   CGG--- 5'
ErhI Erwinia rhaponici B9 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
ErhB9I Erwinia rhaponici B9 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorII
ErhB9II Erwinia rhaponici B9 5' CCWWGG
3' GGWWCC 		5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
ErpI Erwinia rhaponici 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI
EsaBC3I Environmental sample BC3 5' TCGA
3' AGCT 		5' ---TC   GA--- 3'
3' ---AG   CT--- 5'
EsaBC4I Environmental sample BC4 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
EspI Eucapsis sp. 5' GCTNAGC
3' CGANTCG 		5' ---GC   TNAGC--- 3'
3' ---CGANT   CG--- 5'
Esp3I Erwinia sp. RFL3 5' CGTCTC
3' GCAGAG 		5' ---CGTCTCN   NNNN--- 3'
3' ---GCAGAGNNNNN   --- 5'
Esp4I Erwinia sp. RFL4 5' CTTAAG
3' GAATTC 		5' ---C   TTAAG--- 3'
3' ---GAATT   C--- 5'
Esp1396I Enterobacter sp. RFL1396 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I
This list is incomplete; you can help by adding missing items with reliable sources.

F
Enzyme PDB code Source Recognition sequence Cut Isoschizomers
FalI Flavobacterium aquatile Ob10 5' AAGN5CTT
3' TTCN5GAA 		5' ---AAGN5CTTN7NNNNNN   --- 3'
3' ---TTCN5GAAN7N   NNNNN--- 5'
FalII Flavobacterium aquatile Ob10 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI, ThaI
FaqI Flavobacterium aquatile RFL1 5' GGGAC
3' CCCTG 		5' ---GGGACN8NN   NNNN--- 3'
3' ---CCCTGN8NNNNNN   --- 5'
FatI Flavobacterium aquatile NL3 5' CATG
3' GTAC 		5' ---   CATG--- 3'
3' ---GTAC   --- 5'
FauI Flavobacterium aquatile 5' CCCGC
3' GGGCG 		5' ---CCCGCNNNN   NN--- 3'
3' ---GGGCGNNNNNN   --- 5'
FauBII Flavobacterium aureus B 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI, ThaI
FauNDI Flavobacterium aquatile ND 5' CATATG
3' GTATAC 		5' ---CA   TATG--- 3'
3' ---GTAT   AC--- 5'
FbaI Flavobacterium balustinum 5' TGATCA
3' ACTAGT 		5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BclI, BsiQI,
BspXII, BstT7I, Ksp22I,  ParI 
FblI Flavobacterium balustinum 5' GTMKAC
3' CAKMTG 		5' ---GT   MKAC--- 3'
3' ---CAKM   TG--- 5' 		AccI, XmiI
FbrI Flavobacterium breve 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
FdiI Fremyella diplosiphon 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI
FdiII Fremyella diplosiphon 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, AviII, FspI, MstI, NsbI, PamI, Pun14627I
FgoI Fervidobacterium gondwanense AB39T 5' CTAG
3' GATC 		5' ---C   TAG--- 3'
3' ---GAT   C--- 5'
FmuI Flavobacterium multivorum 5' GGNCC
3' CCNGG 		5' ---GGNC   C--- 3'
3' ---C   CNGG--- 5' 		FmuI AvcI, BavAII, Bce22I, Bsu54I, NspIV, Pde12I, UnbI
FnuAI Fusobacterium nucleatum A 5' GANTC
3' CTNAG 		5' ---G   ANTC--- 3'
3' ---CTNA   G--- 5'
FnuCI Fusobacterium nucleatum C 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bme12I, Bsp67I, Bst19II, CcyI, FnuEI, MgoI, NphI, SauMI
FnuDI Fusobacterium nucleatum D 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
FnuDII Fusobacterium nucleatum D 5' CGCG
3' GCGC 		5' ---CG   CG--- 3'
3' ---GC   GC--- 5' 		Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FnuDII, ThaI
FnuDIII Fusobacterium nucleatum D 5' GCGC
3' CGCG 		5' ---GCG   C--- 3'
3' ---C   GCG--- 5' 		AspLEI, BspLAI, BstHHI, CfoI, HhaI, Hin6I, HinP1I, HspAI, SciNI
FnuEI Fusobacterium nucleatum E 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, Sau3AI
Fnu4HI Fusobacterium nucleatum 4H 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
FokI 2FOK Flavobacterium okeanokoites 5' GGATG
3' CCTAC 		5' ---GGATGN8N   NNNN--- 3'
3' ---CCTACN8NNNNN   --- 5'
FriOI Flavobacterium sp. O9 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
FseI Frankia sp. Eu11b 5' GGCCGGCC
3' CCGGCCGG 		5' ---GGCCGG   CC--- 3'
3' ---CC   GGCCGG--- 5'
FsiI Frankia sp. 5' RAATTY
3' YTTAAR 		5' ---R   AATTY--- 3'
3' ---YTTAA   R--- 5'
AcsI, ApoI,  CfaI,  XapI
FspI Fischerella sp. 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, AviII, FdiII, MstI, NsbI, PamI, Pun14627I
FspII Fischerella sp. 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, Ssp1I, SviI
Fsp1604I Flavobacterium sp. I 16-04 5' CCWGG
3' GGWCC 		5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5' 		ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, SleI
FspAI Flexibacter sp. TV-m21K 5' RTGCGCAY
3' YACGCGTR 		5' ---RTGC   GCAY--- 3'
3' ---YACG   CGTR--- 5'
FspBI Flavobacterium sp. RFLI 5' CTAG
3' GATC 		5' ---C   TAG--- 3'
3' ---GAT   C--- 5'
Fsp4HI Flavobacterium sp. 4H 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
FspMSI Fischerella sp. 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, DsaIV, ErpI, HgiBI, HgiEI, Kzo49I, SmuEI
FssI Fibrobacter succinogenes S85 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Kzo49I, VpaK11AI
FunI Fischerella uniformis 5' AGCGCT
3' TCGCGA 		5' ---AGC   GCT--- 3'
3' ---TCG   CGA--- 5' 		AfeI, AitI, Aor51H, Eco47III
FunII Fischerella uniformis 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
This list is incomplete; you can help by adding missing items with reliable sources.

Notes
  1. Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
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