Cyclin E1

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene.[5]

CCNE1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1W98
Identifiers
Aliases	CCNE1, CCNE, pcyclin E1
External IDs	OMIM: 123837 MGI: 88316 HomoloGene: 14452 GeneCards: CCNE1
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19q12 Start 29,811,991 bp[1] End 29,824,312 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 B3|7 25.35 cM Start 38,097,984 bp[2] End 38,107,534 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001105 transcription coactivator activity • kinase activity • cyclin-dependent protein serine/threonine kinase regulator activity • GO:0001948 protein binding • androgen receptor binding • protein kinase binding • cyclin-dependent protein serine/threonine kinase activity • protein kinase activity Cellular component • cytosol • cyclin-dependent protein kinase holoenzyme complex • nucleoplasm • cell nucleus • cyclin E1-CDK2 complex • cytoplasm • centrosome Biological process • androgen receptor signaling pathway • Wnt signaling pathway • regulation of transcription involved in G1/S transition of mitotic cell cycle • regulation of cell cycle • cell division • positive regulation of transcription, DNA-templated • protein phosphorylation • DNA replication initiation • cell cycle • regulation of protein kinase activity • negative regulation of transcription from RNA polymerase II promoter • telomere maintenance • synapsis • chromosome organization involved in meiotic cell cycle • regulation of cellular protein localization • G1/S transition of mitotic cell cycle • regulation of cyclin-dependent protein serine/threonine kinase activity • GO:0007067 mitotic cell cycle • regulation of mitotic nuclear division • positive regulation of cell proliferation • positive regulation of cell cycle • positive regulation of G1/S transition of mitotic cell cycle • mitotic cell cycle phase transition Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	898	12447
Ensembl	ENSG00000105173	ENSMUSG00000002068
UniProt	P24864	Q61457
RefSeq (mRNA)	NM_001238 NM_057182 NM_001322259 NM_001322261 NM_001322262	NM_007633
RefSeq (protein)	NP_001229 NP_001309188 NP_001309190 NP_001309191 NP_001309188.1 NP_001309190.1	NP_031659
Location (UCSC)	Chr 19: 29.81 – 29.82 Mb	Chr 7: 38.1 – 38.11 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.[6]

Interactions

Cyclin E1 has been shown to interact with:

• CDC25A,[7][8]
• CDKN1B,[7][9]
• CUL3[10]
• Cdk1,[7][11]
• Cyclin-dependent kinase 2,[7][9][11][12][13][14]
• HERC5,[15]
• P21,[12]
• Retinoblastoma-like protein 2,[7][16] and
• SMARCA4.[7][17]

See also

• Cyclin E

References

1. GRCh38: Ensembl release 89: ENSG00000105173 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000002068 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lew DJ, Dulić V, Reed SI (October 1991). "Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast". Cell. 66 (6): 1197–206. doi:10.1016/0092-8674(91)90042-W. PMID 1833066. S2CID 12166415.
6. "Entrez Gene: CCNE1 cyclin E1".
7. Shanahan F, Seghezzi W, Parry D, Mahony D, Lees E (February 1999). "Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest". Mol. Cell. Biol. 19 (2): 1460–9. doi:10.1128/mcb.19.2.1460. PMC 116074. PMID 9891079.
8. Xu X, Burke SP (March 1996). "Roles of active site residues and the NH2-terminal domain in the catalysis and substrate binding of human Cdc25". J. Biol. Chem. 271 (9): 5118–24. doi:10.1074/jbc.271.9.5118. PMID 8617791.
9. Connor MK, Kotchetkov R, Cariou S, Resch A, Lupetti R, Beniston RG, Melchior F, Hengst L, Slingerland JM (January 2003). "CRM1/Ran-mediated nuclear export of p27(Kip1) involves a nuclear export signal and links p27 export and proteolysis". Mol. Biol. Cell. 14 (1): 201–13. doi:10.1091/mbc.E02-06-0319. PMC 140238. PMID 12529437.
10. Singer JD, Gurian-West M, Clurman B, Roberts JM (September 1999). "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells". Genes Dev. 13 (18): 2375–87. doi:10.1101/gad.13.18.2375. PMC 317026. PMID 10500095.
11. Koff A, Giordano A, Desai D, Yamashita K, Harper JW, Elledge S, Nishimoto T, Morgan DO, Franza BR, Roberts JM (September 1992). "Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle". Science. 257 (5077): 1689–94. doi:10.1126/science.1388288. PMID 1388288.
12. McKenzie PP, Danks MK, Kriwacki RW, Harris LC (July 2003). "P21Waf1/Cip1 dysfunction in neuroblastoma: a novel mechanism of attenuating G0-G1 cell cycle arrest". Cancer Res. 63 (13): 3840–4. PMID 12839982.
13. Mayer C, Zhao J, Yuan X, Grummt I (February 2004). "mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability". Genes Dev. 18 (4): 423–34. doi:10.1101/gad.285504. PMC 359396. PMID 15004009.
14. Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, Murray M, Krainer AR, Stalmans W, Bollen M (August 2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry". J. Biol. Chem. 275 (33): 25411–7. doi:10.1074/jbc.M001676200. PMID 10827081.
15. Mitsui K, Nakanishi M, Ohtsuka S, Norwood TH, Okabayashi K, Miyamoto C, Tanaka K, Yoshimura A, Ohtsubo M (December 1999). "A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins". Biochem. Biophys. Res. Commun. 266 (1): 115–22. doi:10.1006/bbrc.1999.1777. PMID 10581175.
16. Li Y, Graham C, Lacy S, Duncan AM, Whyte P (December 1993). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E". Genes Dev. 7 (12A): 2366–77. doi:10.1101/gad.7.12a.2366. PMID 8253383.
17. Wong AK, Shanahan F, Chen Y, Lian L, Ha P, Hendricks K, Ghaffari S, Iliev D, Penn B, Woodland AM, Smith R, Salada G, Carillo A, Laity K, Gupte J, Swedlund B, Tavtigian SV, Teng DH, Lees E (November 2000). "BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines". Cancer Res. 60 (21): 6171–7. PMID 11085541.

Further reading

• Akita H (2003). "[Prognostic importance of altered expression of cell cycle regulators in lung cancer]". Nippon Rinsho. 60. Suppl 5: 267–71. PMID 12101670.
• Mazumder S, DuPree EL, Almasan A (2004). "A dual role of cyclin E in cell proliferation and apoptosis may provide a target for cancer therapy". Current Cancer Drug Targets. 4 (1): 65–75. doi:10.2174/1568009043481669. PMC 1307511. PMID 14965268.
• Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA (1992). "Regulation of retinoblastoma protein functions by ectopic expression of human cyclins". Cell. 70 (6): 993–1006. doi:10.1016/0092-8674(92)90249-C. PMID 1388095. S2CID 30799229.
• Koff A, Giordano A, Desai D, Yamashita K, Harper JW, Elledge S, Nishimoto T, Morgan DO, Franza BR, Roberts JM (1992). "Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle". Science. 257 (5077): 1689–94. doi:10.1126/science.1388288. PMID 1388288.
• Koff A, Cross F, Fisher A, Schumacher J, Leguellec K, Philippe M, Roberts JM (1991). "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family". Cell. 66 (6): 1217–28. doi:10.1016/0092-8674(91)90044-Y. PMID 1833068. S2CID 38910227.
• Demetrick DJ, Matsumoto S, Hannon GJ, Okamoto K, Xiong Y, Zhang H, Beach DH (1995). "Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3)". Cytogenet. Cell Genet. 69 (3–4): 190–2. doi:10.1159/000133960. PMID 7698009.
• Ohtsubo M, Theodoras AM, Schumacher J, Roberts JM, Pagano M (1995). "Human cyclin E, a nuclear protein essential for the G1-to-S phase transition". Mol. Cell. Biol. 15 (5): 2612–24. doi:10.1128/mcb.15.5.2612. PMC 230491. PMID 7739542.
• Sewing A, Rönicke V, Bürger C, Funk M, Müller R (1994). "Alternative splicing of human cyclin E.". J. Cell Sci. 107. ( Pt 2): 581–8. PMID 8207080.
• Dyson N, Dembski M, Fattaey A, Ngwu C, Ewen M, Helin K (1993). "Analysis of p107-associated proteins: p107 associates with a form of E2F that differs from pRB-associated E2F-1". J. Virol. 67 (12): 7641–7. doi:10.1128/JVI.67.12.7641-7647.1993. PMC 238233. PMID 8230483.
• Li Y, Graham C, Lacy S, Duncan AM, Whyte P (1994). "The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E." Genes Dev. 7 (12A): 2366–77. doi:10.1101/gad.7.12a.2366. PMID 8253383.
• Xu X, Burke SP (1996). "Roles of active site residues and the NH2-terminal domain in the catalysis and substrate binding of human Cdc25". J. Biol. Chem. 271 (9): 5118–24. doi:10.1074/jbc.271.9.5118. PMID 8617791.
• Ohtani K, DeGregori J, Nevins JR (1996). "Regulation of the cyclin E gene by transcription factor E2F1". Proc. Natl. Acad. Sci. U.S.A. 92 (26): 12146–50. doi:10.1073/pnas.92.26.12146. PMC 40313. PMID 8618861.
• Geng Y, Eaton EN, Picón M, Roberts JM, Lundberg AS, Gifford A, Sardet C, Weinberg RA (1996). "Regulation of cyclin E transcription by E2Fs and retinoblastoma protein". Oncogene. 12 (6): 1173–80. PMID 8649818.
• Poon RY, Jiang W, Toyoshima H, Hunter T (1996). "Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage". J. Biol. Chem. 271 (22): 13283–91. doi:10.1074/jbc.271.22.13283. PMID 8662825.
• Chen J, Saha P, Kornbluth S, Dynlacht BD, Dutta A (1996). "Cyclin-binding motifs are essential for the function of p21CIP1". Mol. Cell. Biol. 16 (9): 4673–82. doi:10.1128/MCB.16.9.4673. PMC 231467. PMID 8756624.
• Li H, Lahti JM, Valentine M, Saito M, Reed SI, Look AT, Kidd VJ (1997). "Molecular cloning and chromosomal localization of the human cyclin C (CCNC) and cyclin E (CCNE) genes: deletion of the CCNC gene in human tumors". Genomics. 32 (2): 253–9. doi:10.1006/geno.1996.0112. PMID 8833152.
• Won KA, Reed SI (1996). "Activation of cyclin E/CDK2 is coupled to site-specific autophosphorylation and ubiquitin-dependent degradation of cyclin E." EMBO J. 15 (16): 4182–93. doi:10.1002/j.1460-2075.1996.tb00793.x. PMC 452142. PMID 8861947.
• Sala A, Kundu M, Casella I, Engelhard A, Calabretta B, Grasso L, Paggi MG, Giordano A, Watson RJ, Khalili K, Peschle C (1997). "Activation of human B-MYB by cyclins". Proc. Natl. Acad. Sci. U.S.A. 94 (2): 532–6. doi:10.1073/pnas.94.2.532. PMC 19547. PMID 9012818.
• Mumberg D, Wick M, Bürger C, Haas K, Funk M, Müller R (1997). "Cyclin ET, a new splice variant of human cyclin E with a unique expression pattern during cell cycle progression and differentiation". Nucleic Acids Res. 25 (11): 2098–105. doi:10.1093/nar/25.11.2098. PMC 146711. PMID 9153308.