Calf-intestinal alkaline phosphatase
Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate.[3] This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
| Calf-intestinal alkaline phosphatase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | ALPI | ||||||
| UniProt | P19111 | ||||||
| |||||||
| Calf-intestinal alkaline phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.1.3.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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References
- Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
- Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM (December 1977). "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone". Nature. 270 (5637): 486–94. Bibcode:1977Natur.270..486S. doi:10.1038/270486a0. PMID 339105. S2CID 4196683.
- Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (June 1977). "Rat insulin genes: construction of plasmids containing the coding sequences". Science. New York, N.Y. 196 (4296): 1313–9. Bibcode:1977Sci...196.1313U. doi:10.1126/science.325648. PMID 325648.
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