Vibrio natriegens

Vibrio natriegens (V. natriegens) is a Gram-negative marine bacterium.[3] It was first isolated from salt marsh mud. It is a salt-loving organism (halophile) requiring about 2% NaCl for growth. It reacts well to the presence of sodium ions which appear to stimulate growth in Vibrio species, to stabilise the cell membrane, and to affect sodium-dependent transport and mobility. Under optimum conditions, and all nutrients provided, the doubling time of V. natriegens can be less than 10 minutes. In the laboratory, the growth medium can be easily changed, thus affecting the growth rate of a culture.[5][6] V. natriegens is commonly found in estuarine mud.

Vibrio natriegens
Scientific classification
Kingdom:
Bacteria
Phylum:
Proteobacteria
Class:
Gammaproteobacteria
Order:
Vibrionales
Family:
Vibrionaceae
Genus:
Vibrio
Binomial name
Vibrio natriegens
Synonyms

Pseudomonas natriegens [1]
Beneckea natriegens [1][4]

Biotechnological uses

Owing to its rapid growth rate, ability to grow on inexpensive carbon sources, and capacity to secrete proteins into the growth media, efforts are underway to leverage this species as a host for molecular biology and biotechnology applications.[7][8] Recently, V. natriegens crude extract has been shown by multiple research groups to be a promising platform for cell-free expression.[9][10][11][12]

References
  1. Payne WJ, Eagon RG, Williams AK (1961). "Some observations on the physiology of Pseudomonas natriegens nov. spec". Antonie van Leeuwenhoek. 27: 121–8. doi:10.1007/bf02538432. PMID 13733692.
  2. Woolkalis MJ, Baumann P (July 1981). "Evolution of alkaline phosphatase in marine species of Vibrio". J. Bacteriol. 147 (1): 36–45. PMC 216004. PMID 6787029.
  3. Baumann P, Baumann L, Bang SS, Woolkalis MJ (1980). "Reevaluation of the taxonomy of Vibrio, Beneckea, and Photobacterium: Abolition of the genus Beneckea". Curr. Microbiol. 4 (3): 127–132. doi:10.1007/bf02602814.
  4. Baumann P, Baumann L, Mandel M (July 1971). "Taxonomy of marine bacteria: the genus Beneckea". J. Bacteriol. 107 (1): 268–94. PMC 246914. PMID 4935323.
  5. Aiyar SE, Gaal T, Gourse RL (Mar 2002). "rRNA promoter activity in the fast-growing bacterium Vibrio natriegens". J. Bacteriol. 184 (5): 1349–58. doi:10.1128/jb.184.5.1349-1358.2002. PMC 134863. PMID 11844764.
  6. R. G. Eagon (1962). "Pseudomonas natriegens, A Marine Bacterium with a Generation Time of Less Than 10 Minutes". J. Bacteriol. 83 (4): 736–737. doi:10.1128/jb.83.4.736-737.1962. PMC 279347. PMID 13888946.
  7. Lee, Henry H.; Ostrov, Nili; Wong, Brandon G.; Gold, Michaela A.; Khalil, Ahmad S.; Church, George M. (2016-06-12). "Vibrio natriegens, a new genomic powerhouse". BioRxiv. doi:10.1101/058487.
  8. Weinstock, Matthew T.; Hesek, Eric D.; Wilson, Christopher M.; Gibson, Daniel G. (2016-08-29). "Vibrio natriegens as a fast-growing host for molecular biology". Nature Methods. 13 (10): 849–51. doi:10.1038/nmeth.3970. ISSN 1548-7105. PMID 27571549.
  9. Wiegand, Daniel J.; Lee, Henry H.; Ostrov, Nili; Church, George M. (2018-09-12). "Establishing a Cell-free Vibrio natriegens Expression System". ACS Synthetic Biology. 7 (10): 2475–2479. doi:10.1021/acssynbio.8b00222. PMID 30160938.
  10. Wiegand, Daniel J.; Lee, Henry H.; Ostrov, Nili; Church, George M. (2019-03-15). "Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens". Journal of Visualized Experiments (145). doi:10.3791/59495. PMC 6512795. PMID 30933074.
  11. Des Doye, BJ; Davidson, SR; Weinstock, MT; Gibson, DG; Jewett, MC (2018-09-06). "Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens". ACS Synthetic Biology. 7 (9): 2245–2255. doi:10.1021/acssynbio.8b00252. PMID 30107122.
  12. Failmezger, J; Scholz, S; Blombach, B; Siemann-Herzberg, M (2018-06-01). "Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens". Frontiers in Microbiology. 9. doi:10.3389/fmicb.2018.01146. PMC 5992293. PMID 29910785.


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