Transformer proteins

Transformer proteins (TFPs) are proteins that can transform from one conformation to a different one and simultaneously change their function.[1]

Protein structures in a given environment were thought to be defined completely by their amino acid sequence.[2] These protein structures are usually related to one single physiological protein activity. This hypothesis was, however, challenged by observations that proteins could fold in two alternative conformations, such as the prion proteins which exist in a physiologically active cellular form and an insoluble form.[3] Extending the concept of a protein that exists in a soluble and an insoluble form, for the bacterial transcription factor RfaH two entirely different structures were observed to coexist in solution.[4] RfaH is a two-domain protein, the C-terminal domain (CTD) of which can fold into alpha-helical and, alternatively, into beta-barrel form. These two interconvertible structures have two different functions, in alpha-helix form the CTD inhibits binding of the N-terminal domain to the RNA-polymerase, whereas the beta-barrel form recruits ribosomes. RfaH is thus the first member of the transformer protein class which obviously violates the original 'one sequence - one structure - one function' suggestion that governed protein research for decades . [5][6][7]

See also
  • Moonlighting protein

References
  1. Emily Caldwell (19 July 2012). "LIKE A TRANSFORMER? PROTEIN UNFOLDS AND REFOLDS FOR NEW FUNCTION". researchnews.osu.edu. Retrieved 14 September 2012.
  2. Anfinsen, Christian B., Robert R. Redfield WI, Choate JP, Carroll WR. Studies on the Gross Structure, Cross-Linkages, and Terminal Sequences in Ribonuclease. J Biological Chem. 207:201-10
  3. Prusiner, S. B.; Scott, M. R.; DeArmond, S. J.; Cohen, F. E. (1998). "Prion protein biology". Cell. 93 (3): 337–48. doi:10.1016/S0092-8674(00)81163-0. PMID 9590169.
  4. Burmann, B. M.; Knauer, S. H.; Sevostyanova, A.; Schweimer, K.; R. Landick, R. M. Mooney; Artsimovitch, I.; Rösch, P. (2012). "An α-Helix to β-Barrel Domain Switch Transforms the Transcription Factor RfaH into a Translation Factor". Cell. 150 (2): 291–303. doi:10.1016/j.cell.2012.05.042. PMC 3430373. PMID 22817892.
  5. Knauer, S. H.; Artsimovitch, I.; Rösch, P. (2012). "Transformer proteins". Cell Cycle. 11 (23): 4289–90. doi:10.4161/cc.22468. PMC 3552902. PMID 23095672.
  6. Knauer, S. H.; Rösch, P.; Artsimovitch, I. (2012). "Transformation: the next level of regulation". RNA Biology. 9 (12): 1418–23. doi:10.4161/rna.22724. PMC 3679274. PMID 23131843.
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