Pinin

Pinin is a protein that in humans is encoded by the PNN gene.[5][6]

PNN
Identifiers
Aliases	PNN, DRS, DRSP, SDK3, memA, Pinin, pinin, desmosome associated protein
External IDs	OMIM: 603154 MGI: 1100514 HomoloGene: 37656 GeneCards: PNN
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q21.1 Start 39,175,183 bp[1] End 39,183,220 bp[1]
Gene location (Mouse) Chr. Chromosome 12 (mouse)[2] Band 12 C1|12 26.0 cM Start 59,066,884 bp[2] End 59,073,998 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • structural molecule activity • DNA binding • RNA binding • GO:0001948 protein binding Cellular component • intermediate filament • cell junction • cell nucleus • intercellular junction • membrane • desmosome • exon-exon junction complex • cell membrane • catalytic step 2 spliceosome • spliceosomal complex • nuclear speck Biological process • mRNA splicing, via spliceosome • transcription, DNA-templated • RNA splicing • mRNA processing • regulation of transcription, DNA-templated • cell adhesion Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5411	18949
Ensembl	ENSG00000100941	ENSMUSG00000020994
UniProt	Q9H307	O35691
RefSeq (mRNA)	NM_002687	NM_008891
RefSeq (protein)	NP_002678	NP_032917
Location (UCSC)	Chr 14: 39.18 – 39.18 Mb	Chr 12: 59.07 – 59.07 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

Pinin has been shown to interact with Keratin 8,[7] Keratin 18,[7] Keratin 19,[7] CTBP1,[8] RNPS1,[9][10] PRPF4B,[11] SFRS4,[12] PPIG,[13] SRRM2[12] and SFRS18.[12]

References

1. GRCh38: Ensembl release 89: ENSG00000100941 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020994 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ouyang P, Sugrue SP (January 1997). "Characterization of pinin, a novel protein associated with the desmosome-intermediate filament complex". J Cell Biol. 135 (4): 1027–42. doi:10.1083/jcb.135.4.1027. PMC 2133386. PMID 8922384.
6. "Entrez Gene: PNN pinin, desmosome associated protein".
7. Shi, J; Sugrue S P (May 2000). "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus". J. Biol. Chem. 275 (20): 14910–5. doi:10.1074/jbc.275.20.14910. ISSN 0021-9258. PMID 10809736.
8. Alpatov, Roman; Munguba Gustavo Costa; Caton Paul; Joo Jeong Hoon; Shi Yang; Shi Yujiang; Hunt Marguerite E; Sugrue Stephen P (December 2004). "Nuclear speckle-associated protein Pnn/DRS binds to the transcriptional corepressor CtBP and relieves CtBP-mediated repression of the E-cadherin gene". Mol. Cell. Biol. 24 (23): 10223–35. doi:10.1128/MCB.24.23.10223-10235.2004. ISSN 0270-7306. PMC 529029. PMID 15542832.
9. Ewing, Rob M; Chu Peter; Elisma Fred; Li Hongyan; Taylor Paul; Climie Shane; McBroom-Cerajewski Linda; Robinson Mark D; O'Connor Liam; Li Michael; Taylor Rod; Dharsee Moyez; Ho Yuen; Heilbut Adrian; Moore Lynda; Zhang Shudong; Ornatsky Olga; Bukhman Yury V; Ethier Martin; Sheng Yinglun; Vasilescu Julian; Abu-Farha Mohamed; Lambert Jean-Philippe; Duewel Henry S; Stewart Ian I; Kuehl Bonnie; Hogue Kelly; Colwill Karen; Gladwish Katharine; Muskat Brenda; Kinach Robert; Adams Sally-Lin; Moran Michael F; Morin Gregg B; Topaloglou Thodoros; Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
10. Li, Chin; Lin Ru-Inn; Lai Ming-Chih; Ouyang Pin; Tarn Woan-Yuh (October 2003). "Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1". Mol. Cell. Biol. 23 (20): 7363–76. doi:10.1128/MCB.23.20.7363-7376.2003. ISSN 0270-7306. PMC 230327. PMID 14517304.
11. Dellaire, Graham; Makarov Evgeny M; Cowger Jeff J M; Longman Dasa; Sutherland Heidi G E; Lührmann Reinhard; Torchia Joseph; Bickmore Wendy A (July 2002). "Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes". Mol. Cell. Biol. 22 (14): 5141–56. doi:10.1128/MCB.22.14.5141-5156.2002. ISSN 0270-7306. PMC 139773. PMID 12077342.
12. Zimowska, Grazyna; Shi Jia; Munguba Gustavo; Jackson Moira R; Alpatov Roman; Simmons Matthew N; Shi Yujiang; Sugrue Stephen P (November 2003). "Pinin/DRS/memA interacts with SRp75, SRm300 and SRrp130 in corneal epithelial cells". Invest. Ophthalmol. Vis. Sci. 44 (11): 4715–23. doi:10.1167/iovs.03-0240. ISSN 0146-0404. PMID 14578391.
13. Lin, Chun Lun; Leu Steve; Lu Ming Chu; Ouyang Pin (August 2004). "Over-expression of SR-cyclophilin, an interaction partner of nuclear pinin, releases SR family splicing factors from nuclear speckles". Biochem. Biophys. Res. Commun. 321 (3): 638–47. doi:10.1016/j.bbrc.2004.07.013. ISSN 0006-291X. PMID 15358154.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Brandner JM, Reidenbach S, Franke WW (1998). "Evidence that "pinin", reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein". Differentiation. 62 (3): 119–27. doi:10.1046/j.1432-0436.1997.6230119.x. PMID 9447706.
• Degen WG, Agterbos MA, Muyrers JP, et al. (1999). "memA/DRS, a putative mediator of multiprotein complexes, is overexpressed in the metastasizing human melanoma cell lines BLM and MV3". Biochim. Biophys. Acta. 1444 (3): 384–94. doi:10.1016/s0167-4781(99)00012-3. PMID 10095061.
• Ouyang P (1999). "Antibodies differentiate desmosome-form and nucleus-form pinin: evidence that pinin is a moonlighting protein with dual location at the desmosome and within the nucleus". Biochem. Biophys. Res. Commun. 263 (1): 192–200. doi:10.1006/bbrc.1999.1353. PMID 10486276.
• Shi Y, Ouyang P, Sugrue SP (2000). "Characterization of the gene encoding pinin/DRS/memA and evidence for its potential tumor suppressor function". Oncogene. 19 (2): 289–97. doi:10.1038/sj.onc.1203328. PMID 10645008.
• Shi J, Sugrue SP (2000). "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus". J. Biol. Chem. 275 (20): 14910–5. doi:10.1074/jbc.275.20.14910. PMID 10809736.
• Hu RM, Han ZG, Song HD, et al. (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
• Wang P, Lou PJ, Leu S, Ouyang P (2002). "Modulation of alternative pre-mRNA splicing in vivo by pinin". Biochem. Biophys. Res. Commun. 294 (2): 448–55. doi:10.1016/S0006-291X(02)00495-3. PMID 12051732.
• Dellaire G, Makarov EM, Cowger JJ, et al. (2002). "Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes". Mol. Cell. Biol. 22 (14): 5141–56. doi:10.1128/MCB.22.14.5141-5156.2002. PMC 139773. PMID 12077342.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Chang WL, Lee DC, Leu S, et al. (2003). "Molecular characterization of a novel nucleolar protein, pNO40". Biochem. Biophys. Res. Commun. 307 (3): 569–77. doi:10.1016/S0006-291X(03)01208-7. PMID 12893261.
• Li C, Lin RI, Lai MC, et al. (2003). "Nuclear Pnn/DRS protein binds to spliced mRNPs and participates in mRNA processing and export via interaction with RNPS1". Mol. Cell. Biol. 23 (20): 7363–76. doi:10.1128/MCB.23.20.7363-7376.2003. PMC 230327. PMID 14517304.
• Zimowska G, Shi J, Munguba G, et al. (2003). "Pinin/DRS/memA interacts with SRp75, SRm300 and SRrp130 in corneal epithelial cells". Invest. Ophthalmol. Vis. Sci. 44 (11): 4715–23. doi:10.1167/iovs.03-0240. PMID 14578391.
• Sakashita E, Tatsumi S, Werner D, et al. (2004). "Human RNPS1 and its associated factors: a versatile alternative pre-mRNA splicing regulator in vivo". Mol. Cell. Biol. 24 (3): 1174–87. doi:10.1128/MCB.24.3.1174-1187.2004. PMC 321435. PMID 14729963.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Lin CL, Leu S, Lu MC, Ouyang P (2004). "Over-expression of SR-cyclophilin, an interaction partner of nuclear pinin, releases SR family splicing factors from nuclear speckles". Biochem. Biophys. Res. Commun. 321 (3): 638–47. doi:10.1016/j.bbrc.2004.07.013. PMID 15358154.