Peptidylprolyl isomerase A

Peptidylprolyl isomerase A (PPIA), also known as cyclophilin A (CypA) or rotamase A is an enzyme that in humans is encoded by the PPIA gene on chromosome 7.[4][5][6] As a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, this protein catalyzes the cis-trans isomerization of proline imidic peptide bonds, which allows it to regulate many biological processes, including intracellular signaling, transcription, inflammation, and apoptosis.[4][7][8][9][10] Due to its various functions, PPIA has been implicated in a broad range of inflammatory diseases, including atherosclerosis and arthritis, and viral infections.[8][9][10]

PPIA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1AK4, 1AWQ, 1AWR, 1AWS, 1AWT, 1AWU, 1AWV, 1BCK, 1CWA, 1CWB, 1CWC, 1CWF, 1CWH, 1CWI, 1CWJ, 1CWK, 1CWL, 1CWM, 1CWO, 1FGL, 1M63, 1M9C, 1M9D, 1M9E, 1M9F, 1M9X, 1M9Y, 1MF8, 1MIK, 1NMK, 1OCA, 1RMH, 1VBS, 1VBT, 1W8L, 1W8M, 1W8V, 1YND, 1ZKF, 2ALF, 2CPL, 2CYH, 2MZU, 2N0T, 2RMA, 2RMB, 2X25, 2X2A, 2X2C, 2X2D, 2XGY, 3CYH, 3CYS, 3K0M, 3K0N, 3K0O, 3K0P, 3K0Q, 3K0R, 3ODI, 3ODL, 3RDD, 4CYH, 4IPZ, 4N1M, 4N1N, 4N1O, 4N1P, 4N1Q, 4N1R, 4N1S, 4YUO, 5CYH, 2MS4, 4YUG, 4YUH, 4YUI, 4YUJ, 4YUK, 4YUL, 4YUM, 4YUN, 4YUP, 5F66, 5FJB
Identifiers
Aliases	PPIA, CYPA, CYPH, HEL-S-69p, Peptidylprolyl isomerase A
External IDs	OMIM: 123840 MGI: 97749 HomoloGene: 134504 GeneCards: PPIA
Gene location (Human) Chr. Chromosome 7 (human)[1] Band 7p13 Start 44,796,680 bp[1] End 44,824,564 bp[1]
Gene ontology Molecular function • GO:0001948 protein binding • virion binding • unfolded protein binding • isomerase activity • peptidyl-prolyl cis-trans isomerase activity • RNA binding • cyclosporin A binding Cellular component • cytoplasm • cell nucleus • membrane • focal adhesion • cytosol • extracellular region • secretory granule lumen • ficolin-1-rich granule lumen • vesicle • extracellular exosome • extracellular • macromolecular complex Biological process • GO:0019067 viral life cycle • positive regulation of protein secretion • regulation of viral genome replication • uncoating of virus • lipid particle organization • virion assembly • leukocyte migration • RNA-dependent DNA biosynthetic process • positive regulation of viral genome replication • viral shedding • establishment of integrated proviral latency • protein peptidyl-prolyl isomerization • protein folding • fusion of virus membrane with host plasma membrane • GO:0022415 viral process • neutrophil degranulation • interleukin-12-mediated signaling pathway • protein refolding Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5478	268373
Ensembl	ENSG00000196262	ENSMUSG00000071866
UniProt	P62937	P17742
RefSeq (mRNA)	NM_203431 NM_001300981 NM_021130 NM_203430	NM_008907
RefSeq (protein)	NP_001287910 NP_066953	NP_032933
Location (UCSC)	Chr 7: 44.8 – 44.82 Mb	n/a
PubMed search	[2]	[3]
Wikidata
View/Edit Human View/Edit Mouse

Structure

PPIA is an 18 kDa, 165-amino acid long cytosolic protein.[11] Like other cyclophilins, PPIA forms a β-barrel structure with a hydrophobic core. This β-barrel is composed of eight anti-parallel β-strands and capped by two α-helices at the top and bottom.[7][11][12] In addition, the β-turns and loops in the strands contribute to the flexibility of the barrel.[12] Its active site is a hydrophobic pocket that binds peptides containing proline. Cyclosporine can bind this pocket to inhibit the protein’s enzymatic activity.[7]

Function

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate protein folding.[4][11] Generally, PPIases are found in all eubacteria and eukaryotes, as well as in a few archaebacteria, and thus are highly conserved.[7][10] Of the 18 known human cyclophilins, PPIA is the most abundantly expressed isozyme.[10] In particular, PPIA is predominantly expressed in the nucleus and cytoplasm of the cell, where it partakes in intracellular signaling, protein transport, and transcription regulation.[7][8][9][10][11] In hemopoietic cells, subcellular localization of PPIA from the nucleus to the cytoplasm has been observed during c-Jun N-terminal kinase- and serine protease-dependent microtubule disruption. This localization has been correlated with G2/M arrest, indicating that the protein's PPIase function may be regulated by microtubule dynamics during the cell cycle.[11] PPIA has also been associated with the mitochondria.[13]

Moreover, the enzyme participates in inflammatory and apoptotic processes in extracellular settings. In the presence of reactive oxygen species (ROS), vascular smooth muscle cells (VSMCs), monocytes/macrophages, and endothelial cells (ECs) secrete PPIA to induce an inflammatory response and mitigate tissue injury.[8][9][11][14] PPIA may also activate Akt and NF-κB signaling, resulting in the upregulation of Bcl-2, an antiapoptotic protein, and thus preventing apoptosis in ECs in response to oxidative stress.[9] PPIA may also regulate ERK1/2, JNK, p38 kinase, Akt, and IκB signalling pathways through activating the CD147 receptor.[11] PPIA-mediated activation of the ERK, JNK, and p38 kinase pathways also contributes to angiogenesis.[11] Additionally, PPIA induces cell migration and proliferation in smooth muscle.[8] In the case of T cells, PPIA regulates the T-cell-specific tyrosine kinase ITK upon T-cell receptor stimulation.[10]

Clinical significance

The PPIA protein is an important apoptotic constituent. During a normal embryologic processes, or during cell injury (such as ischemia-reperfusion injury during heart attacks and strokes) or during developments and processes in cancer, an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and fragmentation of the DNA and nucleus. This is followed by fragmentation into apoptotic bodies that are quickly removed by phagocytes, thereby preventing an inflammatory response.[15] It is a mode of cell death defined by characteristic morphological, biochemical and molecular changes. It was first described as a "shrinkage necrosis", and then this term was replaced by apoptosis to emphasize its role opposite mitosis in tissue kinetics. In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies which contain nuclear and or cytoplasmic elements. The ultrastructural appearance of necrosis is quite different, the main features being mitochondrial swelling, plasma membrane breakdown and cellular disintegration. Apoptosis occurs in many physiological and pathological processes. It plays an important role during embryonal development as programmed cell death and accompanies a variety of normal involutional processes in which it serves as a mechanism to remove "unwanted" cells.

As a proinflammatory cytokine, PPIA is highly involved in acute and chronic inflammatory diseases, including sepsis, atherosclerosis, and rheumatoid arthritis.[8][9][10] Thus, therapeutic targeting of PPIA with selective inhibitors may prove effective in combatting such inflammatory diseases and symptoms.[9][10] Correlation between plasma PPIA levels and hyperglycemia symptoms also promotes utilization of PPIA as a biomarker for diabetes and vascular disease.[8]

Furthermore, PPIA is involved in cerebral hypoxia-ischemia by contributing to the nuclear transport of AIF, a proapoptotic factor, in neurons.[10] To maintain the integrity of the blood brain barrier and mitigate brain injury, PPIA helps to recruit circulating monocytes and stimulates survival and growth pathways.[8] In cardiac myogenic cells, cyclophilins have been observed to be activated by heat shock and hypoxia-reoxygenation as well as complex with heat shock proteins. Thus, cyclophilins may function in cardioprotection during ischemia-reperfusion injury.

Currently, PPIA expression is highly correlated with cancer pathogenesis, but the specific mechanisms remain to be elucidated.[11][14] PPIA overexpression has been associated with hepatocellular carcinoma, lung cancer, pancreatic adenocarcinoma, endometrial carcinoma, esophageal squamous cell carcinoma, and melanoma.[11][13]

The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.[4][16] As a result, PPIA contributes to viral diseases such as AIDS, hepatitis C, measles, and influenza A.[10]

Interactions

Peptidylprolyl isomerase A has been shown to interact with:

• ITK,[17]
• CD147,[8][11]
• P53,[10]
• STAT3,[10]

See also

• Cyclophilin
• TRIM5alpha

References

1. GRCh38: Ensembl release 89: ENSG00000196262 - Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Entrez Gene: PPIA peptidylprolyl isomerase A (cyclophilin A)".
5. Haendler B, Hofer E (Jul 1990). "Characterization of the human cyclophilin gene and of related processed pseudogenes". European Journal of Biochemistry / FEBS. 190 (3): 477–82. doi:10.1111/j.1432-1033.1990.tb15598.x. PMID 2197089.
6. Holzman TF, Egan DA, Edalji R, Simmer RL, Helfrich R, Taylor A, Burres NS (Feb 1991). "Preliminary characterization of a cloned neutral isoelectric form of the human peptidyl prolyl isomerase cyclophilin". The Journal of Biological Chemistry. 266 (4): 2474–9. PMID 1989998.
7. Kazui T, Inoue N, Yamada O, Komatsu S (Jan 1992). "Selective cerebral perfusion during operation for aneurysms of the aortic arch: a reassessment". The Annals of Thoracic Surgery. 53 (1): 109–14. doi:10.1016/0003-4975(92)90767-x. PMID 1530810.
8. Ramachandran S, Venugopal A, Kutty VR, A V, G D, Chitrasree V, Mullassari A, Pratapchandran NS, Santosh KR, Pillai MR, Kartha CC (7 February 2014). "Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease". Cardiovascular Diabetology. 13: 38. doi:10.1186/1475-2840-13-38. PMC 3922405. PMID 24502618.
9. Wei Y, Jinchuan Y, Yi L, Jun W, Zhongqun W, Cuiping W (Jun 2013). "Antiapoptotic and proapoptotic signaling of cyclophilin A in endothelial cells". Inflammation. 36 (3): 567–72. doi:10.1007/s10753-012-9578-7. PMID 23180369. S2CID 24968009.
10. Hoffmann H, Schiene-Fischer C (Jul 2014). "Functional aspects of extracellular cyclophilins". Biological Chemistry. 395 (7–8): 721–35. doi:10.1515/hsz-2014-0125. PMID 24713575. S2CID 32395688.
11. Obchoei S, Wongkhan S, Wongkham C, Li M, Yao Q, Chen C (Nov 2009). "Cyclophilin A: potential functions and therapeutic target for human cancer". Medical Science Monitor. 15 (11): RA221–32. PMID 19865066.
12. Wang T, Yun CH, Gu SY, Chang WR, Liang DC (Aug 2005). "1.88 A crystal structure of the C domain of hCyP33: a novel domain of peptidyl-prolyl cis-trans isomerase". Biochemical and Biophysical Research Communications. 333 (3): 845–9. doi:10.1016/j.bbrc.2005.06.006. PMID 15963461.
13. Ye Y, Huang A, Huang C, Liu J, Wang B, Lin K, Chen Q, Zeng Y, Chen H, Tao X, Wei G, Wu Y (2013). "Comparative mitochondrial proteomic analysis of hepatocellular carcinoma from patients". Proteomics Clin Appl. 7 (5–6): 403–15. doi:10.1002/prca.201100103. PMID 23589362. S2CID 5906425.
14. Yao Q, Li M, Yang H, Chai H, Fisher W, Chen C (Mar 2005). "Roles of cyclophilins in cancers and other organ systems". World Journal of Surgery. 29 (3): 276–80. doi:10.1007/s00268-004-7812-7. PMID 15706440. S2CID 11678319.
15. Kerr JF, Wyllie AH, Currie AR (Aug 1972). "Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics". British Journal of Cancer. 26 (4): 239–57. doi:10.1038/bjc.1972.33. PMC 2008650. PMID 4561027.
16. Agarwal, PK (Aug 2004). "Cis/trans isomerization in HIV-1 capsid protein catalyzed by cyclophilin A: insights from computational and theoretical studies". Proteins. 56 (3): 449–63. doi:10.1002/prot.20135. PMID 15229879. S2CID 19907859.
17. Brazin KN, Mallis RJ, Fulton DB, Andreotti AH (Feb 2002). "Regulation of the tyrosine kinase Itk by the peptidyl-prolyl isomerase cyclophilin A". Proceedings of the National Academy of Sciences of the United States of America. 99 (4): 1899–904. doi:10.1073/pnas.042529199. PMC 122291. PMID 11830645.

Further reading

• Franke EK, Luban J (1995). "Cyclophilin and gag in HIV-1 replication and pathogenesis". Cell Activation and Apoptosis in HIV Infection. Advances in Experimental Medicine and Biology. 374. pp. 217–28. doi:10.1007/978-1-4615-1995-9_19. ISBN 978-0-306-45063-1. PMID 7572395.
• Sokolskaja E, Luban J (Aug 2006). "Cyclophilin, TRIM5, and innate immunity to HIV-1". Current Opinion in Microbiology. 9 (4): 404–8. doi:10.1016/j.mib.2006.06.011. PMID 16815734.