PDE8A

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A is an enzyme that in humans is encoded by the PDE8A gene.[5][6] Work by Sebastiaan Bol et al. showed that 5 different transcript variants and their corresponding isoforms are expressed in human macrophages, and suggests that this protein may be required by HIV-1 for its replication.[7]

PDE8A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3ECM, 3ECN
Identifiers
Aliases	PDE8A, HsT19550, phosphodiesterase 8A
External IDs	OMIM: 602972 MGI: 1277116 HomoloGene: 1957 GeneCards: PDE8A
EC number	3.1.4.53
Gene location (Human) Chr. Chromosome 15 (human)[1] Band 15q25.3 Start 84,980,440 bp[1] End 85,139,145 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 D3 Start 81,213,596 bp[2] End 81,334,533 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • phosphoric diester hydrolase activity • hydrolase activity • 3',5'-cyclic-nucleotide phosphodiesterase activity • metal ion binding • 3',5'-cyclic-AMP phosphodiesterase activity • kinase binding • 3',5'-cyclic-GMP phosphodiesterase activity Cellular component • cytosol • extracellular exosome Biological process • cyclic nucleotide metabolic process • cAMP catabolic process • regulation of transcription, DNA-templated • signal transduction • positive regulation of protein phosphorylation • negative regulation of cell death • positive regulation of ERK1 and ERK2 cascade • cellular response to epidermal growth factor stimulus • negative regulation of hydrogen peroxide-induced cell death • G-protein coupled receptor signaling pathway Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5151	18584
Ensembl	ENSG00000073417	ENSMUSG00000025584
UniProt	O60658	O88502
RefSeq (mRNA)	NM_001243137 NM_002605 NM_173454 NM_173455 NM_173456 NM_173457	NM_008803
RefSeq (protein)	NP_001230066 NP_002596 NP_775656	NP_032829
Location (UCSC)	Chr 15: 84.98 – 85.14 Mb	Chr 7: 81.21 – 81.33 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

References

1. GRCh38: Ensembl release 89: ENSG00000073417 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000025584 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Fisher DA, Smith JF, Pillar JS, St Denis SH, Cheng JB (Jul 1998). "Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase". Biochem Biophys Res Commun. 246 (3): 570–577. doi:10.1006/bbrc.1998.8684. PMID 9618252.
6. "Entrez Gene: PDE8A phosphodiesterase 8A".
7. Bol SM, Booiman T, Bunnik EM, Moerland PD, van Dort K, Strauss JF III, Sieberer M, Schuitemaker H, Kootstra NA, van 't Wout AB (Sep 2011). "Polymorphism in HIV-1 dependency factor PDE8A affects mRNA level and HIV-1 replication in primary macrophages". Virology. 420 (1): 32–42. doi:10.1016/j.virol.2011.08.013. PMID 21920574.

Further reading

• Bass BL (2002). "RNA editing by adenosine deaminases that act on RNA". Annu. Rev. Biochem. 71: 817–846. doi:10.1146/annurev.biochem.71.110601.135501. PMC 1823043. PMID 12045112.
• Zauli G, Milani D, Mirandola P, et al. (2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway". FASEB J. 15 (2): 483–491. doi:10.1096/fj.00-0354com. PMID 11156964.
• Glavas NA, Ostenson C, Schaefer JB, et al. (2001). "T cell activation up-regulates cyclic nucleotide phosphodiesterases 8A1 and 7A3". Proc. Natl. Acad. Sci. U.S.A. 98 (11): 6319–6324. doi:10.1073/pnas.101131098. PMC 33466. PMID 11371644.
• Wang P, Wu P, Egan RW, Billah MM (2002). "Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution". Gene. 280 (1–2): 183–194. doi:10.1016/S0378-1119(01)00783-1. PMID 11738832.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Gamanuma M, Yuasa K, Sasaki T, et al. (2003). "Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans". Cell. Signal. 15 (6): 565–574. doi:10.1016/S0898-6568(02)00146-8. PMID 12681444.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–45. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wu P, Wang P (2005). "Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins". Proc. Natl. Acad. Sci. U.S.A. 101 (51): 17634–17639. doi:10.1073/pnas.0407649101. PMC 539753. PMID 15596729.