Nuclear cap-binding protein complex

Nuclear cap-binding protein complex is a RNA-binding protein which binds to the 5' cap of pre-mRNA. The cap and nuclear cap-binding protein have many functions in mRNA biogenesis including splicing, 3'-end formation by stabilizing the interaction of the 3'-end processing machinery, nuclear export and protection of the transcripts from nuclease degradation.[2] When RNA is exported to the cytoplasm the nuclear cap-binding protein complex is replaced by cytoplasmic cap binding complex. The nuclear cap-binding complex is a functional heterodimer and composed of Cbc1/Cbc2 in yeast and CBC20/CBC80 in multicellular eukaryotes. Human nuclear cap-binding protein complex shows the large subunit, CBC80 consists of 757 amino acid residues. Its secondary structure contains approximately sixty percent of helical and one percent of beta sheet in the strand. The small subunit, CBC20 has 98 amino acid residues. Its secondary structure contains approximately twenty percent of helical and twenty-four percent of beta sheet in the strand.[1] Human nuclear cap-binding protein complex plays important role in the maturation of pre-mRNA and uracil-rich small nuclear RNA.[3]

nuclear cap-binding protein complex
Crystal structure of the human nuclear cap-binding complex.[1]
Identifiers
SymbolNCBP1
Alt. symbolsNCBP
NCBI gene4686
HGNC7658
RefSeqNM_002486
Other data
LocusChr. 9 q34.1
Nuclear cap binding protein subunit 2, 20kDa
Identifiers
SymbolNCBP2
NCBI gene22916
HGNC7659
OMIM605133
RefSeqNM_007362
UniProtP52298
Other data
LocusChr. 3 q29

References
  1. PDB: 1H6K​; Mazza C, Ohno M, Segref A, Mattaj IW, Cusack S (August 2001). "Crystal structure of the human nuclear cap binding complex". Mol. Cell. 8 (2): 383–96. doi:10.1016/S1097-2765(01)00299-4. PMID 11545740.
  2. Raczynska KD, Simpson CG, Ciesiolka A, et al. (January 2010). "Involvement of the nuclear cap-binding protein complex in alternative splicing in Arabidopsis thaliana". Nucleic Acids Res. 38 (1): 265–78. doi:10.1093/nar/gkp869. PMC 2800227. PMID 19864257.
  3. Mazza C, Segref A, Mattaj IW, Cusack S (October 2002). "Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex". EMBO J. 21 (20): 5548–57. doi:10.1093/emboj/cdf538. PMC 129070. PMID 12374755.


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