NBPF15

NBPF15
Identifiers
Aliases	NBPF15, AB14, AG3, NBPF16, neuroblastoma breakpoint family member 15, NBPF member 15, MGC8902
External IDs	OMIM: 610414, 614005 HomoloGene: 41035 GeneCards: NBPF15
Gene location (Human)
Chr.	Chromosome 1 (human)[1]
End	144,461,674 bp[1]
Orthologs
	284565
	n/a
	ENSG00000266338
	n/a
	Q8N660
	n/a
	NM_001102663; NM_001170755; NM_173638
	n/a
	NP_001164226; NP_775909
	n/a
	Wikidata
View/Edit Human

Neuroblastoma breakpoint family, member 15, also known as NBPF15, is a protein which in humans is encoded by the NBPF15 gene.[3] The gene is 18762 bp long, with mRNA that is 3837 bp long. The gene is located on chromosome 1q21.1. Its sub-cellular location is predicted to be in the nucleus and cytoplasm.[4] It contains what is known as the NBPF repeat, which is a two-exon stretch of sequence that is characteristic of all 21 members of the NBPF gene family. The repeat is considered the ancestral exons, and the NBPF family has been linked to primate evolution.[5]

Function

The function of NBPF16 is not fully understood. It is a member of the NBPF family of proteins, which have been linked to possible roles in oncogenesis and tumor suppressor genes.[5]

Protein

The protein is composed of 670 amino acids. The gene contains five domains of unknown function, called DUF1220. DUF1220 domains are found in all members of the NBPF gene family, although the number differs between each member. Repetitive structure with high intergenic and intragenic sequence conservation, both in coding and noncoding regions. Makes it possible for homologous recombination to occur easily between different alleles. The repetitiveness of it, and the other members of the NBPF gene family is thought to have arisen from segmental duplications on chromosome 1.[5]

Predicted properties

Properties of NBPF16 that were predicted using bioinformatics tools:

Molecular weight: 76 kD[6]
Isoelectric point: 4.43[7]
Post-translational modification: none predicted.
No predicted signal peptide or signal peptide cleavage site.[8]
No interacting proteins or binding partners.

Expression

There is little to no expression data available for the gene, but most indications point to it being ubiquitously expressed throughout the body.

Homology

Orthologs

There exists no great orthologs outside of primates. These orthologs were gathered from BLAT.[9] and BLAST searches[10]

Species	Organism common name	Sequence identity	Sequence similarity	Length (AAs)
Homo sapiens	Human	100%	100%	670
Pan troglodytes	Chimpanzee	90%	92%	509
Macaca mulatta	Rhesus macaque	57%	68%	620
Gallus gallus	Chicken	36%	54%	1394
Rattus norvegicus	Norway rat	34%	67%	2324
Bos taurus	Cattle	34%	50%	816
Xenopus laevis	African clawed frog	33%	52%	728
Mus musculus	House mouse	32%	55%	2446

Paralogs

Due to there being 21 other members of the NBPF gene family, there are 21 paralogs of NBPF16. They all show high conservation and repetitive structures.

References

GRCh38: Ensembl release 89: ENSG00000266338 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Entrez Gene: NBPF16 neuroblastoma breakpoint family, member 16". Retrieved 13 May 2009.
"SDSC Biology Workbench". San Diego Supercomputer Center. Retrieved 13 May 2009.
Vandepoele K, Van Roy N, Staes K, Speleman F, van Roy F (August 2005). "A novel gene family NBPF: intricate structure generated by gene duplications during primate evolution". Molecular Biology and Evolution. 22 (11): 2265–2274. doi:10.1093/molbev/msi222. PMID 16079250.
Brendel V, Bucher P, Nourbakhsh IR, Blaisdell BE, Karlin S (March 1992). "Methods and algorithms for statistical analysis of protein sequences". Proceedings of the National Academy of Sciences of the United States of America. 89 (6): 2002–6. Bibcode:1992PNAS...89.2002B. doi:10.1073/pnas.89.6.2002. PMC 48584. PMID 1549558.
"PI Program (Isoelectric Point Prediction)". Archived from the original on 26 October 2008.
Bendtsen JD, Nielsen H, von Heijne G, Brunak S (July 2004). "Improved prediction of signal peptides: SignalP 3.0". Journal of Molecular Biology. 340 (4): 783–95. CiteSeerX 10.1.1.165.2784. doi:10.1016/j.jmb.2004.05.028. PMID 15223320.
"BLAT Search Genome". Retrieved 13 May 2009.
"BLAST". Retrieved 13 May 2009.