MED8

Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene.[5][6][7]

MED8
Identifiers
Aliases	MED8, ARC32, mediator complex subunit 8
External IDs	OMIM: 607956 MGI: 1915269 HomoloGene: 10560 GeneCards: MED8
Gene location (Human) Chr. Chromosome 1 (human)[1] Band 1p34.2 Start 43,383,917 bp[1] End 43,389,808 bp[1]
Gene location (Mouse) Chr. Chromosome 4 (mouse)[2] Band 4 D2.1|4 Start 118,409,337 bp[2] End 118,415,782 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001104 transcription coregulator activity • GO:0001948 protein binding • GO:0000980 RNA polymerase II cis-regulatory region sequence-specific DNA binding Cellular component • mediator complex • cell nucleus • nucleoplasm • core mediator complex Biological process • regulation of transcription from RNA polymerase II promoter • transcription initiation from RNA polymerase II promoter • regulation of transcription, DNA-templated • protein ubiquitination • transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	112950	80509
Ensembl	ENSG00000159479	ENSMUSG00000006392
UniProt	Q96G25	Q9D7W5
RefSeq (mRNA)	NM_201542 NM_001001653 NM_052877	NM_001290688 NM_020000 NM_173719
RefSeq (protein)	NP_001001653 NP_443109 NP_963836	NP_001277617 NP_064384 NP_776067
Location (UCSC)	Chr 1: 43.38 – 43.39 Mb	Chr 4: 118.41 – 118.42 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Five alternative transcripts encoding four isoforms have been described.[7]

Interactions

MED8 has been shown to interact with MED26.[8]

References

1. GRCh38: Ensembl release 89: ENSG00000159479 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000006392 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC (Aug 2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase". Proc Natl Acad Sci U S A. 99 (16): 10353–8. Bibcode:2002PNAS...9910353B. doi:10.1073/pnas.162424199. PMC 124918. PMID 12149480.
6. Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD (Aug 1998). "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways". Proc Natl Acad Sci U S A. 95 (15): 8538–43. Bibcode:1998PNAS...95.8538J. doi:10.1073/pnas.95.15.8538. PMC 21111. PMID 9671713.
7. "Entrez Gene: MED8 mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)".
8. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.

Further reading

• Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R (1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature. 398 (6730): 828–32. Bibcode:1999Natur.398..828N. doi:10.1038/19789. PMID 10235267. S2CID 23646963.
• Xu XR, Huang J, Xu ZG, Qian BZ, Zhu ZD, Yan Q, Cai T, Zhang X, Xiao HS, Qu J, Liu F, Huang QH, Cheng ZH, Li NG, Du JJ, Hu W, Shen KT, Lu G, Fu G, Zhong M, Xu SH, Gu WY, Huang W, Zhao XT, Hu GX, Gu JR, Chen Z, Han ZG (2002). "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 15089–94. doi:10.1073/pnas.241522398. PMC 64988. PMID 11752456.
• Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". J. Biol. Chem. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
• Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
• Léonard VH, Kohl A, Hart TJ, Elliott RM (2006). "Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response". J. Virol. 80 (19): 9667–75. doi:10.1128/JVI.00822-06. PMC 1617248. PMID 16973571.