MED6

Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene.[5][6][7]

MED6
Identifiers
Aliases	MED6, ARC33, NY-REN-28, mediator complex subunit 6
External IDs	OMIM: 602984 MGI: 1917042 HomoloGene: 3990 GeneCards: MED6
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q24.2 Start 70,581,257 bp[1] End 70,600,690 bp[1]
Gene location (Mouse) Chr. Chromosome 12 (mouse)[2] Band 12|12 D1 Start 81,573,557 bp[2] End 81,595,008 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001105 transcription coactivator activity • GO:0001104 transcription coregulator activity • transcription factor binding • GO:0001948 protein binding Cellular component • mediator complex • core mediator complex • membrane • cell nucleus • nucleoplasm Biological process • regulation of transcription from RNA polymerase II promoter • RNA polymerase II transcriptional preinitiation complex assembly • transcription initiation from RNA polymerase II promoter • regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10001	69792
Ensembl	ENSG00000133997	ENSMUSG00000002679
UniProt	O75586	Q921D4
RefSeq (mRNA)	NM_005466 NM_001284209 NM_001284210 NM_001284211	NM_027213 NM_001347384 NM_001347385 NM_001347386
RefSeq (protein)	NP_001271138 NP_001271139 NP_001271140 NP_005457	NP_001334313 NP_001334314 NP_001334315
Location (UCSC)	Chr 14: 70.58 – 70.6 Mb	Chr 12: 81.57 – 81.6 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Med6
Identifiers
Symbol	Med6
Pfam	PF04934
InterPro	IPR007018
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Protein family

This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.[5]

Interactions

MED6 has been shown to interact with:

• Cyclin-dependent kinase 8,[8][9][10][11]
• Estrogen receptor alpha,[11]
• MED21,[12] and
• Thyroid hormone receptor alpha.[10]

References

1. GRCh38: Ensembl release 89: ENSG00000133997 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000002679 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Lee YC, Min S, Gim BS, Kim YJ (August 1997). "A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans". Mol. Cell. Biol. 17 (8): 4622–32. doi:10.1128/MCB.17.8.4622. PMC 232315. PMID 9234719.
6. Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD (Aug 1998). "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways". Proc Natl Acad Sci U S A. 95 (15): 8538–8543. Bibcode:1998PNAS...95.8538J. doi:10.1073/pnas.95.15.8538. PMC 21111. PMID 9671713.
7. "Entrez Gene: MED6 mediator of RNA polymerase II transcription, subunit 6 homolog (S. cerevisiae)".
8. Yang F, DeBeaumont R, Zhou S, Näär AM (Feb 2004). "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator". Proc. Natl. Acad. Sci. U.S.A. 101 (8): 2339–44. Bibcode:2004PNAS..101.2339Y. doi:10.1073/pnas.0308676100. PMC 356952. PMID 14983011.
9. Wang G, Cantin GT, Stevens JL, Berk AJ (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Mol. Cell. Biol. 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. PMC 87123. PMID 11416138.
10. Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–70. doi:10.1016/s1097-2765(00)80463-3. PMID 10198638.
11. Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2642–7. Bibcode:2002PNAS...99.2642K. doi:10.1073/pnas.261715899. PMC 122401. PMID 11867769.
12. Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (Aug 1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell. 2 (2): 213–22. doi:10.1016/s1097-2765(00)80131-8. PMID 9734358.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–174. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell. 2 (2): 213–222. doi:10.1016/S1097-2765(00)80131-8. PMID 9734358.
• Gu W, Malik S, Ito M, Yuan CX, Fondell JD, Zhang X, Martinez E, Qin J, Roeder RG (1999). "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation". Mol. Cell. 3 (1): 97–108. doi:10.1016/S1097-2765(00)80178-1. PMID 10024883.
• Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG (1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell. 3 (3): 361–370. doi:10.1016/S1097-2765(00)80463-3. PMID 10198638.
• Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Näär AM, Erdjument-Bromage H, Tempst P, Freedman LP (1999). "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex". Nature. 398 (6730): 824–828. Bibcode:1999Natur.398..824R. doi:10.1038/19783. PMID 10235266. S2CID 204992765.
• Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R (1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature. 398 (6730): 828–832. Bibcode:1999Natur.398..828N. doi:10.1038/19789. PMID 10235267. S2CID 23646963.
• Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ (1999). "Antigens recognized by autologous antibody in patients with renal-cell carcinoma". Int. J. Cancer. 83 (4): 456–464. doi:10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5. PMID 10508479.
• Ge K, Guermah M, Yuan CX, Ito M, Wallberg AE, Spiegelman BM, Roeder RG (2002). "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis". Nature. 417 (6888): 563–567. Bibcode:2002Natur.417..563G. doi:10.1038/417563a. PMID 12037571. S2CID 4432077.
• Kato Y, Habas R, Katsuyama Y, Näär AM, He X (2002). "A component of the ARC/Mediator complex required for TGF beta/Nodal signalling". Nature. 418 (6898): 641–646. Bibcode:2002Natur.418..641K. doi:10.1038/nature00969. PMID 12167862. S2CID 4330754.
• Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". J. Biol. Chem. 278 (17): 15123–15127. doi:10.1074/jbc.C300054200. PMID 12584197.
• Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". J. Biol. Chem. 278 (50): 49671–49674. doi:10.1074/jbc.C300444200. PMID 14576168.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". J. Biol. Chem. 279 (7): 5846–5851. doi:10.1074/jbc.M312523200. PMID 14638676.
• Yang F, DeBeaumont R, Zhou S, Näär AM (2004). "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator". Proc. Natl. Acad. Sci. U.S.A. 101 (8): 2339–2344. Bibcode:2004PNAS..101.2339Y. doi:10.1073/pnas.0308676100. PMC 356952. PMID 14983011.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685–691. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
• Pavri R, Lewis B, Kim TK, Dilworth FJ, Erdjument-Bromage H, Tempst P, de Murcia G, Evans R, Chambon P, Reinberg D (2005). "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator". Mol. Cell. 18 (1): 83–96. doi:10.1016/j.molcel.2005.02.034. PMID 15808511.

External links

• MED6 human gene location in the UCSC Genome Browser.
• MED6 human gene details in the UCSC Genome Browser.

This article incorporates text from the public domain Pfam and InterPro: IPR007018