MED28

Mediator of RNA polymerase II transcription subunit 28 is an enzyme that in humans is encoded by the MED28 gene.[5][6][7] It forms part of the Mediator complex.

MED28
Identifiers
Aliases	MED28, 1500003D12Rik, EG1, magicin, mediator complex subunit 28
External IDs	OMIM: 610311 MGI: 1914249 HomoloGene: 11873 GeneCards: MED28
Gene location (Human) Chr. Chromosome 4 (human)[1] Band 4p15.32 Start 17,614,641 bp[1] End 17,634,105 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 B3 Start 45,520,229 bp[2] End 45,529,276 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • actin binding • GO:0001948 protein binding Cellular component • cytoplasm • mediator complex • cytoskeleton • membrane • cell nucleus • cortical actin cytoskeleton • nucleoplasm Biological process • stem cell population maintenance • regulation of transcription, DNA-templated • negative regulation of smooth muscle cell differentiation • transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	80306	66999
Ensembl	ENSG00000118579	ENSMUSG00000015804
UniProt	Q9H204	Q920D3
RefSeq (mRNA)	NM_025205	NM_025895
RefSeq (protein)	NP_079481	NP_080171
Location (UCSC)	Chr 4: 17.61 – 17.63 Mb	Chr 5: 45.52 – 45.53 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Med28
Identifiers
Symbol	Med28
Pfam	PF11594
InterPro	IPR021640
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Function

Subunit Med28 of the Mediator may function as a scaffolding protein within Mediator by maintaining the stability of a submodule within the head module, and components of this submodule act together in a gene-regulatory programme to suppress smooth muscle cell differentiation. Thus, mammalian Mediator subunit Med28 functions as a repressor of smooth muscle-cell differentiation, which could have implications for disorders associated with abnormalities in smooth muscle cell growth and differentiation, including atherosclerosis, asthma, hypertension, and smooth muscle tumours.[8]

Interactions

MED28 has been shown to interact with Merlin,[6] Grb2[6] and MED26.[9]

See also

• Mediator complex

References

1. GRCh38: Ensembl release 89: ENSG00000118579 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000015804 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu C, Zhang L, Shao ZM, Beatty P, Sartippour M, Lane TF, Barsky SH, Livingston E, Nguyen M (Jan 2002). "Identification of a novel endothelial-derived gene EG-1". Biochemical and Biophysical Research Communications. 290 (1): 602–12. doi:10.1006/bbrc.2001.6119. PMID 11779215.
6. Wiederhold T, Lee MF, James M, Neujahr R, Smith N, Murthy A, Hartwig J, Gusella JF, Ramesh V (Nov 2004). "Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2". Oncogene. 23 (54): 8815–25. doi:10.1038/sj.onc.1208110. PMID 15467741.
7. "Entrez Gene: MED28 mediator of RNA polymerase II transcription, subunit 28 homolog (S. cerevisiae)".
8. Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. doi:10.1074/jbc.M706592200. PMID 17848560.
9. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.

This article incorporates text from the public domain Pfam and InterPro: IPR021640

Further reading

• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
• Lu M, Zhang L, Maul RS, Sartippour MR, Norris A, Whitelegge J, Rao JY, Brooks MN (Jul 2005). "The novel gene EG-1 stimulates cellular proliferation". Cancer Research. 65 (14): 6159–66. doi:10.1158/0008-5472.CAN-04-4016. PMID 16024617.
• Lee MF, Beauchamp RL, Beyer KS, Gusella JF, Ramesh V (Sep 2006). "Magicin associates with the Src-family kinases and is phosphorylated upon CD3 stimulation". Biochemical and Biophysical Research Communications. 348 (3): 826–31. doi:10.1016/j.bbrc.2006.07.126. PMID 16899217.
• Lu M, Zhang L, Sartippour MR, Norris AJ, Brooks MN (Oct 2006). "EG-1 interacts with c-Src and activates its signaling pathway". International Journal of Oncology. 29 (4): 1013–8. doi:10.3892/ijo.29.4.1013. PMID 16964398.
• Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V (Nov 2007). "Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation". The Journal of Biological Chemistry. 282 (44): 32152–7. doi:10.1074/jbc.M706592200. PMID 17848560.