Kojibiose phosphorylase

In enzymology, a kojibiose phosphorylase (EC 2.4.1.230) is an enzyme that catalyzes the chemical reaction

2-alpha-D-glucosyl-D-glucose + phosphate D-glucose + beta-D-glucose 1-phosphate
Kojibiose phosphorylase
Identifiers
EC number2.4.1.230
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Thus, the two substrates of this enzyme are kojibiose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is 2-alpha-D-glucosyl-D-glucose:phosphate beta-D-glucosyltransferase.

References

    • Sugimoto T, Kurimoto M, Tsujisaka Y (1999). "Purification and characterization of a novel phosphorylase, kojibiose phosphorylase, from Thermoanaerobium brockii". J. Appl. Glycosci. 46: 423–429.
    • Chaen H, Nishimoto T, Nakada T, Fukuda S, Kurimoto M, Tsujisaka Y (2001). "Enzymatic synthesis of kojioligosaccharides using kojibiose phosphorylase". J. Biosci. Bioeng. 92 (2): 177–82. doi:10.1263/jbb.92.177. PMID 16233080.


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