Hydroxystilbamidine

Hydroxystilbamidine is a fluorescent dye that emits different frequencies of light when bound to DNA and RNA. It is used as a retrograde tracer[1] for outlining neurons, and as a histochemical stain.

Hydroxystilbamidine

Names
IUPAC name 4-[(E)-2-(4-carbamimidoylphenyl)ethenyl]-3-hydroxybenzenecarboximidamide
Identifiers
CAS Number	495-99-8
3D model (JSmol)	Interactive image
ChEMBL	ChEMBL1301
ChemSpider	10612853
ECHA InfoCard	100.007.102
PubChem CID	5353676
UNII	39J262E49W
CompTox Dashboard (EPA)	DTXSID3023136
InChI InChI=1S/C16H16N4O/c17-15(18)12-5-2-10(3-6-12)1-4-11-7-8-13(16(19)20)9-14(11)21/h1-9,21H,(H3,17,18)(H3,19,20)/b4-1+ Key: TUESWZZJYCLFNL-DAFODLJHSA-N InChI=1/C16H16N4O/c17-15(18)12-5-2-10(3-6-12)1-4-11-7-8-13(16(19)20)9-14(11)21/h1-9,21H,(H3,17,18)(H3,19,20)/b4-1+ Key: TUESWZZJYCLFNL-DAFODLJHBK
SMILES Oc2cc(ccc2/C=C/c1ccc(cc1)C(N)=N)C(N)=N
Properties
Chemical formula	C16H16N4O
Molar mass	280.324 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

PVH neurons of Long-Evans rat marked with fluoro-gold

References

1. Naumann, Thomas (2000). "Retrograde tracing with Fluoro-Gold: different methods of tracer detection at the ultrastructural level and neurodegenerative changes of back-filled neurons in long-term studies". Journal of Neuroscience Methods. 103 (1): 11–21. doi:10.1016/S0165-0270(00)00292-2. PMID 11074092.