Hol118 family

The Listeria Phage A118 Holin (Hol118) Family (TC# 1.E.21) is a group of transporters belonging to the Holin Superfamily V.[1] A representative list of proteins belonging to the Hol118 family can be found in the Transporter Classification Database.

Listeria monocytogenes bacteriophage A118 encodes a native holin, hol118, of 93 amino acyl residues (aas) exhibiting 3 putative transmembrane segments (TMSs). When cloned into lambda phage devoid of the S holin, it caused very late cell lysis, beginning 80 min. after induction.[2] Hol118 appeared in the cytoplasmic membrane shortly after infection. A second translational start codon (AUG-3) at nucleotide position 40 in the gene gives rise to a second gene product of 83 aas lacking TMS-1 (Hol118(83)). It is produced with the full length protein, appears in the membrane, but cannot support lambda lysis and inhibits lysis by Hol118. This dominant inhibitor presumably determines the time of lysis.[2] A homologue is found in L. innocua.

The reaction probably catalyzed is:

small molecules + autolysin (in) → small molecules + autolysin (out)

See also

Further reading
  • EMBL-EBI, InterPro. "Bacteriophage A118, holin (IPR009708) < InterPro < EMBL-EBI". www.ebi.ac.uk. Retrieved 2016-03-21.
  • Kuo, Cheng-Yi; Sinha, Shubhra; Jazayeri, Jalal A.; Pouton, Colin W. (2009). "A stably engineered, suicidal strain of listeria monocytogenes delivers protein and/or DNA to fully differentiated intestinal epithelial monolayers". Molecular Pharmaceutics. 6 (4): 1052–1061. doi:10.1021/mp800153u. PMID 19317435.

References
  1. Reddy, Bhaskara L.; Saier Jr., Milton H. (2013-11-01). "Topological and phylogenetic analyses of bacterial holin families and superfamilies". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1828 (11): 2654–2671. doi:10.1016/j.bbamem.2013.07.004. PMC 3788059. PMID 23856191.
  2. Vukov, Natasa; Moll, Isabella; Bläsi, Udo; Scherer, Siegfried; Loessner, Martin J. (2003-04-01). "Functional regulation of the Listeria monocytogenes bacteriophage A118 holin by an intragenic inhibitor lacking the first transmembrane domain". Molecular Microbiology. 48 (1): 173–186. doi:10.1046/j.1365-2958.2003.03421.x. ISSN 0950-382X. PMID 12657053. S2CID 8779394.

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