HIS3
The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.[1] It is analogous to hisB in Escherichia coli.
| Imidazoleglycerol-phosphate dehydratase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | HIS3 | ||||||
| Entrez | 854377 | ||||||
| HomoloGene | 6979 | ||||||
| RefSeq (mRNA) | NM_001183621.1 | ||||||
| RefSeq (Prot) | NP_014845.1 | ||||||
| UniProt | P06633 | ||||||
| Other data | |||||||
| EC number | 4.2.1.19 | ||||||
| Chromosome | XV: 0.72 - 0.72 Mb | ||||||
| |||||||
Exploits
Mutations in Escherichia coli's analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place.[2]
References
- "HIS3/YOR202W Summary". Archived from the original on 2006-05-05. Retrieved 2006-11-30.
- Joung JK, Ramm EI, Pabo CO (June 2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions". Proceedings of the National Academy of Sciences of the United States of America. 97 (13): 7382–7. Bibcode:2000PNAS...97.7382J. doi:10.1073/pnas.110149297. PMC 16554. PMID 10852947.
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