H37Rv

Mycobacterium tuberculosis strain H37Rv is the most studied strain of tuberculosis in research laboratories.[1] It was first isolated by Dr. Edward R. Baldwin in 1905.[2] The strain came from a 19 year old patient with chronic pulmonary tuberculosis at the Trudeau Sanatorium in Saranac Lake, New York.[3] It was maintained for many years by serial passage of cultures at the Trudeau Sanatorium and initially named strain H37. Over time it was found to have variable virulence in animal models based on which medium it was grown on. Strains with different virulence were then intentionally produced, with H37R being less virulent after growing in acidic media and H37S was more virulent in guinea pigs after being grown in alkaline media (with R standing for resistant to environment, and S for sensitive to environment).[3][4] The more virulent strain was later renamed H37Rv, with R standing for rough morphology and v standing for virulent.[4] The strain was used for many laboratory studies and became the standard for tuberculosis. It was later designated as the neotype for the species.[2] Koch first discovered mycobacterium tuberculosis as the cause of tuberculosis in 1892 but the strains he studied were not preserved and it is unclear how related H37Rv may be to those strains. H37Rv has continued to be the strain of tuberculosis most used in laboratories, and was the first to have its complete genome published in 1998.[5] It is unclear how much H37Rv may have evolved in more than 100 years under artificial conditions in laboratories from strains in the wild, but its genome is similar to a strain isolated from a 19th century grave in Yorkshire.[6] However it does not have some characteristics, such as causing caseous necrosis in rabbits, that are seen in modern clinical isolates.[7] Strains in different labs that derive from H37Rv have also been shown to have evolved differences over time, with one survey of 6 strains finding from 5 to 10 polymorphisms per strain.[8] These included independent insertions and deletions of IS6110 transposable elements which would change the strain's spoligotype.[8] The authors of the study cautioned against considering all strains labeled as H37Rv as a reference since there may be significant differences based on the laboratory in which it is maintained.[8]

References
  1. Camus, Jean-Christophe; Médigue, Claudine; Cole, Stewart T.; Pryor, Melinda J. (2002-10-01). "Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv". Microbiology. 148 (10): 2967–2973. doi:10.1099/00221287-148-10-2967. PMID 12368430.
  2. KUBICA, G. P.; KIM, T. H.; DUNBAR, F. P. (1972-04-01). "Designation of Strain H37Rv as the Neotype of Mycobacterium tuberculosis". International Journal of Systematic Bacteriology. 22 (2): 99–106. doi:10.1099/00207713-22-2-99.
  3. Steenken, W.; Oatway, W. H.; Petroff, S. A. (1934-09-30). "Biological Studies of the Tubercle Bacillus: III. Dissociation and Pathogenicity of the R and S Variants of the Human Tubercle Bacillus (H37)". The Journal of Experimental Medicine. 60 (4): 515–540. doi:10.1084/jem.60.4.515. ISSN 0022-1007. PMC 2132400. PMID 19870319.
  4. Steenken, W. (1935). "Lysis of Tubercle Bacilli in Vitro". Experimental Biology and Medicine. 33 (2): 253–255. doi:10.3181/00379727-33-8330p.
  5. Cole, S. T.; Brosch, R.; Parkhill, J.; Garnier, T.; Churcher, C.; Harris, D.; Gordon, S. V.; Eiglmeier, K.; Gas, S. (1998-06-11). "Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence". Nature. 393 (6685): 537–544. Bibcode:1998Natur.393..537C. doi:10.1038/31159. ISSN 0028-0836. PMID 9634230.
  6. Bouwman, Abigail S.; Kennedy, Sandra L.; Müller, Romy; Stephens, Richard H.; Holst, Malin; Caffell, Anwen C.; Roberts, Charlotte A.; Brown, Terence A. (2012-11-06). "Genotype of a historic strain of Mycobacterium tuberculosis". Proceedings of the National Academy of Sciences. 109 (45): 18511–18516. Bibcode:2012PNAS..10918511B. doi:10.1073/pnas.1209444109. ISSN 0027-8424. PMC 3494915. PMID 23091009.
  7. Shammari, Basim Al; Shiomi, Takayuki; Tezera, Liku; Bielecka, Magdalena K.; Workman, Victoria; Sathyamoorthy, Tarangini; Mauri, Francesco; Jayasinghe, Suwan N.; Robertson, Brian D. (2015-08-01). "The Extracellular Matrix Regulates Granuloma Necrosis in Tuberculosis". Journal of Infectious Diseases. 212 (3): 463–473. doi:10.1093/infdis/jiv076. ISSN 0022-1899. PMC 4539912. PMID 25676469.
  8. Ioerger, Thomas R.; Feng, Yicheng; Ganesula, Krishna; Chen, Xiaohua; Dobos, Karen M.; Fortune, Sarah; Jacobs, William R.; Mizrahi, Valerie; Parish, Tanya (2010-07-15). "Variation among Genome Sequences of H37Rv Strains of Mycobacterium tuberculosis from Multiple Laboratories". Journal of Bacteriology. 192 (14): 3645–3653. doi:10.1128/JB.00166-10. ISSN 0021-9193. PMC 2897344. PMID 20472797.
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