Guinea pig maximisation test

The Guinea pig maximisation test (GPMT) is an in vivo test to screen for substances that cause human skin sensitisation (i.e. allergens). It was first proposed by B. Magnusson and Albert Kligman in 1969[1] and described in their 1970 book Allergic Contact Dermatitis in the Guinea Pig[2]

The test animals are exposed intradermally to the test material, along with an adjuvant to enhance the immune reaction of the guinea pig. The guinea pigs are then a short while later exposed to a lower concentration of the test material, and their allergic reaction, if any, measured. 15% of guinea pigs must show a reaction for the test to be considered positive. 20 animals would typically be used to ensure against false negative results.

The OECD Guidelines for the Testing of Chemicals guideline No. 406 of 1992.[3]

The test has been largely superseded by the murine local lymph node assay. D.A. Basketter and E.W. Scholes reviewed them in 1992[4]

The REACH Regulation, Annex VII, paragraph 8.3 states "The Murine Local Lymph Node Assay (LLNA) is the first-choice method for in vivo testing. Only in exceptional circumstances should another test be used. Justification for the use of another test shall be provided."

References

Magnusson B. and Kligman A.M. (1969). The identification of contact allergens by animal assay. The guinea pig maximisation test. Journal of Investigative Dermatology., 52, 268.
Magnusson B. and Kligman A.M. (1970). Allergic Contact Dermatitis in the Guinea Pig. Charles G. Thomas; Springfield, Illinois.
http://www.oecd-ilibrary.org/environment/test-no-406-skin-sensitisation_9789264070660-en
D.A. Basketter and E.W. Scholes (1992) Comparison of the local lymph node assay with the guinea-pig maximization test for the detection of a range of contact allergens Food and Chemical Toxicology Vol 30 pp 65-69

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[1] Magnusson B. and Kligman A.M. (1969). The identification of contact allergens by animal assay. The guinea pig maximisation test. Journal of Investigative Dermatology., 52, 268.

[2] Magnusson B. and Kligman A.M. (1970). Allergic Contact Dermatitis in the Guinea Pig. Charles G. Thomas; Springfield, Illinois.

[3] ttp://www.oecd-ilibrary.org/environment/test-no-406-skin-sensitisation_9789264070660-en

[4] D.A. Basketter and E.W. Scholes (1992) Comparison of the local lymph node assay with the guinea-pig maximization test for the detection of a range of contact allergens Food and Chemical Toxicology Vol 30 pp 65-69