Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (EC 2.4.1.288, GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Identifiers
EC number2.4.1.288
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

This enzyme is solated from Mycobacterium tuberculosis.

References

  1. Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL (August 2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydrate Research. 343 (12): 2130–9. doi:10.1016/j.carres.2008.03.023. PMID 18423586.
  2. May JF, Splain RA, Brotschi C, Kiessling LL (July 2009). "A tethering mechanism for length control in a processive carbohydrate polymerization". Proceedings of the National Academy of Sciences of the United States of America. 106 (29): 11851–6. Bibcode:2009PNAS..10611851M. doi:10.1073/pnas.0901407106. PMC 2715488. PMID 19571009.
  3. Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK (August 2012). "Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan". The Journal of Biological Chemistry. 287 (33): 28132–43. doi:10.1074/jbc.M112.347484. PMC 3431634. PMID 22707726.
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