Electropherogram
An electropherogram, or electrophoregram, is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of molecular biology or biochemistry.[1]
Screenshot of a chromatogram inside the program "Sequencher"
Generation of results
In the field of genetics, an electropherogram is a plot of DNA fragment sizes, typically used for genotyping such as DNA sequencing.[2] Such plots are often achieved using an instrument such as an automated DNA sequencer. Such electropherograms may be used to determine DNA sequence genotypes, or genotypes that are based on the length of specific DNA fragments. These genotypes can be used for:
See also
References
- Karabiber, F (2013). "A peak alignment algorithm with novel improvements in application to electropherogram analysis". Journal of Bioinformatics and Computational Biology. 11 (5): 1350011. doi:10.1142/S021972001350011X. PMC 4529286. PMID 24131055.
- Schwartz, H. and Guttman, A., 1995. Separation of DNA by capillary electrophoresis. Beckman.
External links
- PHPH — web-based tool for electropherogram quality analysis
- Systematic differences in electropherogram peak heights reported by different versions of the GeneScan Software
- DYS464 Electropherogram Interpretation Discrepancy with images
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