DBR1

Lariat debranching enzyme is a protein that in humans is encoded by the DBR1 gene.[5][6]

DBR1
Identifiers
Aliases	DBR1, debranching RNA lariats 1
External IDs	OMIM: 607024 MGI: 1931520 HomoloGene: 9428 GeneCards: DBR1
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3q22.3 Start 138,160,988 bp[1] End 138,174,921 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9|9 E3.3 Start 99,575,799 bp[2] End 99,584,501 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • hydrolase activity • RNA lariat debranching enzyme activity • hydrolase activity, acting on ester bonds • metal ion binding • RNA binding Cellular component • cell nucleus • nucleoplasm Biological process • RNA splicing, via transesterification reactions • mRNA splicing, via spliceosome • mRNA processing • RNA phosphodiester bond hydrolysis, endonucleolytic Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	51163	83703
Ensembl	ENSG00000138231	ENSMUSG00000032469
UniProt	Q9UK59	Q923B1
RefSeq (mRNA)	NM_016216	NM_031403 NM_001368298
RefSeq (protein)	NP_057300	NP_113580 NP_001355227
Location (UCSC)	Chr 3: 138.16 – 138.17 Mb	Chr 9: 99.58 – 99.58 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.[supplied by OMIM][6]

References

1. GRCh38: Ensembl release 89: ENSG00000138231 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032469 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kim JW, Kim HC, Kim GM, Yang JM, Boeke JD, Nam K (Sep 2000). "Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants". Nucleic Acids Res. 28 (18): 3666–73. doi:10.1093/nar/28.18.3666. PMC 110720. PMID 10982890.
6. "Entrez Gene: DBR1 debranching enzyme homolog 1 (S. cerevisiae)".

Further reading

• Chapman KB, Boeke JD (1991). "Isolation and characterization of the gene encoding yeast debranching enzyme". Cell. 65 (3): 483–92. doi:10.1016/0092-8674(91)90466-C. PMID 1850323. S2CID 39225964.
• Arenas J, Hurwitz J (1987). "Purification of a RNA debranching activity from HeLa cells". J. Biol. Chem. 262 (9): 4274–9. PMID 2435736.
• Martin A, Schneider S, Schwer B (2002). "Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome". J. Biol. Chem. 277 (20): 17743–50. doi:10.1074/jbc.M200762200. PMID 11886864.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ye Y, De Leon J, Yokoyama N, et al. (2006). "DBR1 siRNA inhibition of HIV-1 replication". Retrovirology. 2: 63. doi:10.1186/1742-4690-2-63. PMC 1266399. PMID 16232320.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.