Cupiennius salei

Cupiennius salei, commonly called the tiger wandering spider, is a large spider belonging to a group of wandering spiders found in Central America (also one of many diverse types that mistakenly get called banana spiders[1]), although this species is specifically from Eastern Mexico, Guatemala, Belize and Honduras. The species was accidentally introduced into Germany in the early 20th century from banana plantations in Central America. In the mid-1950s it was realised that the spider is an ideal model for biological research because of its large size, inactive behaviour, and ease of breeding in laboratories. From an initial 1963 publication on its biological characteristics, it has become the most studied species of spider.[2][3] Furthermore, the spider is now known to produce a complex neurotoxic venom, such as cupiennins and CSTX, of which a peptide called CsTx-1 is highly potent for paralysing its prey.[4][5] Its toxin has also become one of the most studied among those of venomous spiders. As the spider does not produce a web for trapping prey, being venomous is its primary strategy for prey capture. It is known to prey on a wide range of insects and small vertebrates.

Cupiennius salei
Cupiennius salei juvenile in northern Honduras, by Stuart J. Longhorn
Scientific classification
Kingdom: Animalia
Phylum: Arthropoda
Subphylum: Chelicerata
Class: Arachnida
Order: Araneae
Infraorder: Araneomorphae
Family: Trechaleidae
Genus: Cupiennius
Species:
C. salei
Binomial name
Cupiennius salei
Synonyms

Ctenus salei Keyserling
Ancylometes ahrensi Lucas
Ctenus mordicus F.O.P.-Cambridge
Ctenus oculatus Simon
Cupiennius ahrensi Schmidt
Phoneutria oculifera Karsch

Description
C. salei adult male from Parque Nacional Cusuco, Dept. Cortés, Honduras
C. salei adult female from Parque Nacional Cusuco, Dept. Cortés, Honduras

Cupiennius salei is a large spider with distinct sexual dimorphism. The females are relatively larger than the males, measuring up to 3.5 cm in body length, with a 10 cm legspan. The dorsal side of the body is chocolate-brown with small, lighter spots on the abdomen and many darker longitudinal stripes, particularly on the carapace. The ventral side is red-orange with thick black central region under the abdomen. Males measure up to 2.5 cm long and have very long and thin legs. The males are much lighter in colour than the females. They are distinct, with conspicuous palpal bulbs.[6]

Cupiennius salei have one pair of principal eyes and three pairs of secondary eyes located on the prosoma (the anterior end of the head) and they are colour blind.[7] Being adapted to nocturnality, their visual capability is reduced and they rely on their tactile sensation to detect movements or vibrations in their environment.[6]

Under laboratory conditions, females make cocoons every three to four weeks, each containing up to 1,500 embryos. The embryo is typically 1.3 mm in diameter. The complete life cycle takes between 9 and 12 months from fertilized egg to mature adult. After hatching from the cocoon, the larvae start feeding. Generally they are fed first with fruit flies and later with crickets. They become reproductively mature after the final moult.[3]

Behavior

In the wild, Cupiennius salei are arboreal, living in trees and bushes, favoring plants such as banana with broad flat leaves and wide joints that provide shelter. Although they tend to generally be inactive, they are much faster than many other arachnids when provoked. Cupiennius salei is a nocturnal, "sit-and-wait" ambush predator behaving with a strong circadian rhythmicity. They hide during daylight, mostly under leaves, and emerge at dusk. At sunset, when the light intensity falls to about 15 lux, they leave their refuge but remain nearby. They remain there motionless for about 30 mins until it is dark (less than 0.1 lux) then move to the surface of the leaf and lie in-wait for prey, occasionally briefly walking about the leaf. Their activity is greatest during the first three hours of the night. They retreat back to their refuge after six to seven hours.[8][9]

During mating

During courtship, Cupiennius salei communicate using sex pheromones. Females are usually solitary and to attract males, release the pheromones on trees along a silk thread. When a male detects the pheromone, he shows patterned oscillatory movement that creates vibrations on the leaves (an average frequency of 76 Hz). The female responds to this by creating a counter vibration, and in this way guides the receptive male to her exact location.[10] The female pheromone has been identified as (S)-1,1'-dimethyl citrate. The male's pheromone sensory cells are located in tip pore sensilla and respond to touching with either female silk or the synthetic compound of the pheromone.[11]

Venom

Cupiennius salei produces a neurotoxic venom which is composed of a complex mixture of compounds. The venom contains at least 286 compounds and 49 novel proteins.[12] In addition, there are many low molecular compounds, nine neurotoxic acting peptides (CSTX), at least eight neurotoxic and cytolytic acting peptides (collectively called cupiennins), highly active hyaluronidases.[2] The most powerful neurotoxin is a peptide called CsTx-1.[4] In 2002 a new family of peptides called cupiennins (cupiennin 1a, cupiennin 1b, cupiennin 1c, cupiennin 1d) was discovered from the venom. These proteins are all composed of 35 amino acid residues, and have high antimicrobial activities.[13] It was subsequently discovered that the cupiennins are broad-spectrum bioactive compounds having bactericidal, insecticidal and haemolytic activities.[14]

Venom gland

Cupiennius salei produces its venom in a pair of cylindrical pouch-like glands located at the anterior end of the head (prosoma). In adult females, each gland measures 1.8 mm in diameter and 6.5 mm in length. The glands are connected to a small duct through which the venom is discharged via its fang-like chelicera. Just before entering the chelicera, the duct enlarges to a muscle-invested ampulla and then constricts again. This specific arrangement is believed to be the regulatory system on the amount of venom that is released.[2][15]

Venom optimization
Main article: Venom optimization hypothesis

Cupiennius salei is a non-web producing spider and therefore depends entirely on its venom for predation. It is known to prey on a variety of insects including butterflies, moths, earwigs, cockroaches, flies, grasshoppers and small vertebrates such as frogs and lizards.[16] Its venom glands store only about 10 μl of crude venom.[17] Refilling of the glands takes 2–3 days and the lethal efficacy of the venom is very low for several days after envenomation, requiring 8 to 18 days to regain full effect.[18] It has been determined that the amount of venom released differs between types of prey. For larger and stronger insects such as beetles, the spider uses the entire amount of its venom; for smaller prey, it uses only small amounts, thus economising use of the biologically costly venom.[17][19]

  • Second instar of C. salei (scale bar = 500 μm)
  • C. salei adult female in ventral view. From Cusuco National Park, Dept. Cortés, Honduras

References
  1. Vetter RS, Crawford RL, Buckle DJ (2014). "Spiders (Araneae) Found in Bananas and Other International Cargo Submitted to North American Arachnologists for Identification". Journal of Medical Entomology. 51: 1136–1143. doi:10.1603/me14037. PMID 26309299.
  2. Kuhn-Nentwig L, Schaller J, Nentwig W (2004). "Biochemistry, toxicology and ecology of the venom of the spider Cupiennius salei (Ctenidae)". Toxicon. 43 (5): 543–553. doi:10.1016/j.toxicon.2004.02.009. PMID 15066412.
  3. McGregor AP, Hilbrant M, Pechmann M, Schwager EE, Prpic NM, Damen WG (2008). "Cupiennius salei and Achaearanea tepidariorum: Spider models for investigating evolution and development". BioEssays. 30 (5): 487–498. doi:10.1002/bies.20744. PMID 18404731.
  4. Kuhn-Nentwig L, Fedorova IM, Lüscher BP, Kopp LS, Trachsel C, Schaller J, Vu XL, Seebeck T, Streitberger K, Nentwig W, Sigel E, Magazanik LG (2012). "A venom-derived neurotoxin, CsTx-1, from the spider Cupiennius salei exhibits cytolytic activities". J Biol Chem. 287 (30): 25640–25649. doi:10.1074/jbc.M112.339051. PMC 3408166. PMID 22613721.
  5. Prpic NM, Schoppmeier M, Damen WG (2008). "The American Wandering Spider Cupiennius salei". CSH Protoc. 2008: pdb.emo103. doi:10.1101/pdb.emo103. PMID 21356686.
  6. Francesco Tomasinelli. "Cupiennius salei" (PDF). The British Tarantula Society Journal. Retrieved 14 September 2013.
  7. Orlando E, Schmid A (2011). "Colour blindness of the movement-detecting system of the spider Cupiennius salei". J Exp Biol. 214 (Pt 4): 546–550. doi:10.1242/jeb.051672. PMID 21270302.
  8. Seyfarth EA (1980). "Daily patterns of locomotor activity in a wandering spider". Physiological Entomology. 5 (2): 199–206. doi:10.1111/j.1365-3032.1980.tb00227.x.
  9. Friedrich G. Barth (2002). A Spider's World: Senses and Behavior. Springer-Verlag Berlin Heidelberg. pp. 21–25. ISBN 9783540420460.
  10. Rovner JS, Barth FG (1981). "Vibratory communication through living plants by a tropical wandering spider". Science. 214 (4519): 464–465. doi:10.1126/science.214.4519.464. PMID 17730248.
  11. Tichy H, Gingl E, Ehn R, Papke M, Schulz S (2001). "Female sex pheromone of a wandering spider (Cupiennius salei): identification and sensory reception". J Comp Physiol A. 187 (1): 75–78. doi:10.1007/s003590000175. PMID 11318380.
  12. Trachsel C, Siegemund D, Kämpfer U, Kopp LS, Bühr C, Grossmann J, Lüthi C, Cunningham M, Nentwig W, Kuhn-Nentwig L, Schürch S, Schaller J (2012). "Multicomponent venom of the spider Cupiennius salei: a bioanalytical investigation applying different strategies". FEBS J. 279 (15): 2683–2694. doi:10.1111/j.1742-4658.2012.08650.x. PMID 22672445.
  13. Kuhn-Nentwig L, Muller J, Schaller J, Walz A, Dathe M, Nentwig W (2002). "Cupiennin 1, a new family of highly basic antimicrobial peptides in the venom of the spider Cupiennius salei (Ctenidae)". J Biol Chem. 277 (13): 11208–11216. doi:10.1074/jbc.M111099200. PMID 11792701.
  14. Kuhn-Nentwig L, Sheynis T, Kolusheva S, Nentwig W, Jelinek R (2013). "N-terminal aromatic residues closely impact the cytolytic activity of cupiennin 1a, a major spider venom peptide". Toxicon. 75: 177–86. doi:10.1016/j.toxicon.2013.03.003. PMID 23523532.
  15. Boevé JL, Kuhn-Nentwig L, Keller S, Nentwig W (1995). "Quantity and quality of venom released by a spider (Cupiennius salei, Ctenidae)". Toxicon. 33 (10): 1347–1357. doi:10.1016/0041-0101(95)00066-U. PMID 8599185.
  16. Nentwig W (1986). "Non-webbuilding spiders: prey specialists or generalists?". Oecologia. 69 (4): 571–576. doi:10.1007/BF00410365.
  17. Wigger E, Kuhn-Nentwig L, Nentwig W (2002). "The venom optimisation hypothesis: a spider injects large venom quantities only into difficult prey types". Toxicon. 40 (6): 749–752. doi:10.1016/S0041-0101(01)00277-X. PMID 12175611.
  18. Boevé JL, Kuhn-Nentwig L, Keller S, Nentwig W (1995). "Quantity and quality of venom released by a spider (Cupiennius salei, Ctenidae)". Toxicon. 33 (10): 1347–1357. doi:10.1016/0041-0101(95)00066-U. PMID 8599185.
  19. Morgenstern D, King GF (2013). "The venom optimization hypothesis revisited". Toxicon. 63: 120–128. doi:10.1016/j.toxicon.2012.11.022. PMID 23266311.
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