Chromosome jumping

Chromosome jumping is a tool of molecular biology that is used in the physical mapping of genomes. It is related to several other tools used for the same purpose, including chromosome walking.

Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot be easily mapped by chromosome walking, and is useful in moving along a chromosome rapidly in search of a particular gene.

Chromosome jumping allows more rapid movement through the genome compared to other techniques, such as chromosome walking, and can be used to generate genomic markers with known chromosomal locations.[1]

Chromosome jumping enables two ends of a DNA sequence to be cloned without the middle section. Genomic DNA may be partially digested using restriction endonucleases and with the aid of DNA ligase, the fragments are circularized.[2] From a known sequence, a primer is designed to sequence across the circularised junction. This primer is used to jump 100 kb-300 kb intervals: a sequence 100 kb away would have come near the known sequence on circularisation. Thus, sequences not reachable by chromosome walking can be sequenced. Chromosome walking can be used from the new jump position (in either direction) to look for gene-like sequences, or additional jumps can be used to progress further along the chromosome.

See also

References
  1. Drumm, Mitchell (May 2001). Unit 5.4 Construction of Chromosome Jumping and Linking Libraries in E.coli. Current Protocols in Human Genetics. Chapter 5. pp. Unit 5.4. doi:10.1002/0471142905.hg0504s01. ISBN 978-0471142904. PMID 18428292.
  2. Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer Netherlands. 2008-04-25. pp. 354. ISBN 978-1-4020-6753-2.


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