CTR9

RNA polymerase-associated protein CTR9 homolog is an enzyme that in humans is encoded by the CTR9 gene.[5][6][7]

CTR9
Identifiers
Aliases	CTR9, SH2BP1, TSBP, p150, p150TSP, CTR9 homolog, Paf1/RNA polymerase II complex component
External IDs	OMIM: 609366 MGI: 109345 HomoloGene: 40668 GeneCards: CTR9
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p15.4 Start 10,751,246 bp[1] End 10,801,625 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7 E3|7 57.98 cM Start 111,028,951 bp[2] End 111,056,377 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • RNA polymerase II core binding • SH2 domain binding • GO:0001948 protein binding Cellular component • nuclear speck • transcriptionally active chromatin • cell nucleus • nucleoplasm • Cdc73/Paf1 complex Biological process • histone monoubiquitination • JAK-STAT cascade • negative regulation of mRNA polyadenylation • regulation of transcription, DNA-templated • histone H3-K4 trimethylation • negative regulation of transcription from RNA polymerase II promoter • Wnt signaling pathway • transcription, DNA-templated • stem cell population maintenance • interleukin-6-mediated signaling pathway • endodermal cell fate commitment • positive regulation of histone H3-K4 methylation • inner cell mass cell differentiation • trophectodermal cell differentiation • positive regulation of histone H3-K79 methylation • blastocyst growth • histone H2B ubiquitination • positive regulation of histone H2B ubiquitination • cellular response to lipopolysaccharide • negative regulation of myeloid cell differentiation • regulation of genetic imprinting • positive regulation of transcription elongation from RNA polymerase II promoter • positive regulation of transcription from RNA polymerase II promoter • transcription from RNA polymerase II promoter • transcription elongation from RNA polymerase II promoter • protein ubiquitination • histone modification • regulation of histone H3-K4 methylation • blastocyst hatching Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9646	22083
Ensembl	ENSG00000198730	ENSMUSG00000005609
UniProt	Q6PD62	Q62018
RefSeq (mRNA)	NM_014633 NM_001346279	NM_009431
RefSeq (protein)	NP_001333208 NP_055448	NP_033457
Location (UCSC)	Chr 11: 10.75 – 10.8 Mb	Chr 7: 111.03 – 111.06 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Model organisms

Model organisms have been used in the study of CTR9 function. A conditional knockout mouse line called Ctr9^{tm1b(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.[8] Male and female animals underwent a standardized phenotypic screen[9] to determine the effects of deletion.[10][11][12][13] Additional screens performed: - In-depth immunological phenotyping[14]

Ctr9 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[9][14]
Peripheral blood leukocytes 6 Weeks	Normal
Insulin	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. GRCh38: Ensembl release 89: ENSG00000198730 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000005609 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N (Mar 1996). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (4): 167–74, 199–210. doi:10.1093/dnares/2.4.167. PMID 8590280.
6. Malek SN, Yang CH, Earnshaw WC, Kozak CA, Desiderio S (Jul 1996). "p150TSP, a conserved nuclear phosphoprotein that contains multiple tetratricopeptide repeats and binds specifically to SH2 domains". J Biol Chem. 271 (12): 6952–62. doi:10.1074/jbc.271.12.6952. PMID 8636124.
7. "Entrez Gene: CTR9 Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)".
8. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
9. "International Mouse Phenotyping Consortium".
10. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
11. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
12. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
13. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
14. "Infection and Immunity Immunophenotyping (3i) Consortium".

External links

• Human CTR9 genome location and CTR9 gene details page in the UCSC Genome Browser.

Further reading

• Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243. S2CID 14294292.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.