CNOT1

CCR4-NOT transcription complex subunit 1 is a protein that in humans is encoded by the CNOT1 gene.[5][6]

CNOT1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4C0D, 4CQO, 4CRV, 4CRW, 4CT4, 4CT6, 4CT7, 4GMJ, 4GML, 4J8S, 5ANR, 5FU6, 5FU7
Identifiers
Aliases	CNOT1, CDC39, NOT1, NOT1H, AD-005, CCR4-NOT transcription complex subunit 1, HPE12
External IDs	OMIM: 604917 MGI: 2442402 HomoloGene: 9453 GeneCards: CNOT1
Gene location (Human) Chr. Chromosome 16 (human)[1] Band 16q21 Start 58,519,951 bp[1] End 58,629,885 bp[1]
Gene location (Mouse) Chr. Chromosome 8 (mouse)[2] Band 8|8 D1 Start 95,719,451 bp[2] End 95,807,464 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • poly(A)-specific ribonuclease activity • protein domain specific binding • GO:0001948 protein binding • retinoic acid receptor binding • armadillo repeat domain binding • GO:0032947 molecular adaptor activity • estrogen receptor binding • RNA binding Cellular component • membrane • CCR4-NOT complex • peroxisomal membrane • P-body • CCR4-NOT core complex • cell nucleus • extracellular • cytoplasm • cytosol Biological process • nuclear-transcribed mRNA poly(A) tail shortening • negative regulation of translation • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening • regulation of transcription, DNA-templated • negative regulation of retinoic acid receptor signaling pathway • regulation of stem cell population maintenance • negative regulation of transcription from RNA polymerase II promoter • positive regulation of mRNA catabolic process • transcription, DNA-templated • multicellular organism development • positive regulation of cytoplasmic mRNA processing body assembly • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • gene silencing by miRNA • gene silencing by RNA • negative regulation of intracellular estrogen receptor signaling pathway • regulation of translation • RNA phosphodiester bond hydrolysis, exonucleolytic • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23019	234594
Ensembl	ENSG00000125107	ENSMUSG00000036550
UniProt	A5YKK6 H3BNB1	Q6ZQ08
RefSeq (mRNA)	NM_001265612 NM_016284 NM_206999	NM_001205226 NM_153164 NM_178078 NM_001372351 NM_001372353
RefSeq (protein)	NP_001252541 NP_057368 NP_996882	NP_001192155 NP_694804 NP_835179 NP_001359280 NP_001359282
Location (UCSC)	Chr 16: 58.52 – 58.63 Mb	Chr 8: 95.72 – 95.81 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

CCR4-Not complex component, Not1
Identifiers
Symbol	Not1
Pfam	PF04054
InterPro	IPR007196
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

It is a part of the CCR4-Not complex, which deadenylates mRNAs.[7] CNOT1 acts as a scaffold protein, binding other subunits of the complex.[8][9][10]

Interactions

CNOT1 has been shown to interact with CNOT8.[11][12]

References

1. GRCh38: Ensembl release 89: ENSG00000125107 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000036550 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, et al. (January 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nature Genetics. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
6. "Entrez Gene: CNOT1 CCR4-NOT transcription complex, subunit 1".
7. Garneau NL, Wilusz J, Wilusz CJ (Feb 2007). "The highways and byways of mRNA decay". Nat. Rev. Mol. Cell Biol. 8 (2): 113–26. doi:10.1038/nrm2104. PMID 17245413. S2CID 14107577.
8. Sandler H, Kreth J, Timmers HT, Stoecklin G (May 2011). "Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin". Nucleic Acids Res. 39 (10): 4373–86. doi:10.1093/nar/gkr011. PMC 3105394. PMID 21278420.
9. Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O (Nov 2012). "The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex". Nucleic Acids Res. 40 (21): 11058–72. doi:10.1093/nar/gks883. PMC 3510486. PMID 22977175.
10. Basquin J, Roudko VV, Rode M, Basquin C, Séraphin B, Conti E (Oct 26, 2012). "Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction". Mol. Cell. 48 (2): 207–18. doi:10.1016/j.molcel.2012.08.014. PMID 22959269.
11. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
12. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (February 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63–70. doi:10.1093/dnares/6.1.63. PMID 10231032.
• Chen J, Rappsilber J, Chiang YC, Russell P, Mann M, Denis CL (2001). "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex". J. Mol. Biol. 314 (4): 683–94. doi:10.1006/jmbi.2001.5162. PMID 11733989.
• Laveder P, De Pittà C, Toppo S, Valle G, Lanfranchi G (2002). "A two-step strategy for constructing specifically self-subtracted cDNA libraries". Nucleic Acids Res. 30 (9): 38e–38. doi:10.1093/nar/30.9.e38. PMC 113861. PMID 11972353.
• Winkler GS, Mulder KW, Bardwell VJ, Kalkhoven E, Timmers HT (2006). "Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription". EMBO J. 25 (13): 3089–99. doi:10.1038/sj.emboj.7601194. PMC 1500986. PMID 16778766.

External links

• Eukaryotic Linear Motif resource motif class LIG_CNOT1_NIM_1
• Human CNOT1 genome location and CNOT1 gene details page in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: A5YKK6 (CCR4-NOT transcription complex subunit 1) at the PDBe-KB.