CLASP1

Cytoplasmic linker associated protein 1, also known as CLASP1, is a protein which in humans is encoded by the CLASP1 gene.[5]

CLASP1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4K92
Identifiers
Aliases	CLASP1, MAST1, cytoplasmic linker associated protein 1
External IDs	OMIM: 605852 MGI: 1923957 HomoloGene: 41024 GeneCards: CLASP1
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2q14.2-q14.3 Start 121,337,776 bp[1] End 121,649,587 bp[1]
Gene location (Mouse) Chr. Chromosome 1 (mouse)[2] Band 1|1 E2.3 Start 118,389,058 bp[2] End 118,612,678 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • kinetochore binding • dystroglycan binding • microtubule binding • microtubule plus-end binding • GO:0001948 protein binding Cellular component • cytoplasm • condensed chromosome kinetochore • cytosol • centrosome • Golgi apparatus • kinetochore microtubule • membrane • cortical microtubule cytoskeleton • focal adhesion • spindle • centrosomal corona • chromosome • microtubule plus-end • microtubule organizing center • cell cortex • spindle microtubule • cytoplasmic microtubule • basal cortex • extracellular exosome • chromosome, centromeric region • cytoskeleton • microtubules • kinetochore Biological process • establishment of epithelial cell polarity • vesicle targeting • Golgi organization • GO:0043148 mitotic spindle organization • regulation of epithelial to mesenchymal transition • regulation of microtubule cytoskeleton organization • positive regulation of epithelial cell migration • exit from mitosis • cell division • establishment or maintenance of cell polarity • positive regulation of extracellular matrix disassembly • positive regulation of basement membrane assembly involved in embryonic body morphogenesis • negative regulation of microtubule depolymerization • mitotic spindle assembly • microtubule anchoring • G2/M transition of mitotic cell cycle • microtubule bundle formation • negative regulation of microtubule polymerization or depolymerization • positive regulation of microtubule polymerization • regulation of focal adhesion assembly • negative regulation of wound healing, spreading of epidermal cells • cell cycle • positive regulation of exocytosis • negative regulation of stress fiber assembly • establishment of spindle orientation • astral microtubule organization • microtubule organizing center organization • establishment of mitotic spindle localization • regulation of gastrulation • microtubule cytoskeleton organization • microtubule nucleation • sister chromatid cohesion • ciliary basal body docking • regulation of G2/M transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23332	76707
Ensembl	ENSG00000074054	ENSMUSG00000064302
UniProt	Q7Z460	Q80TV8
RefSeq (mRNA)	NM_001142273 NM_001142274 NM_001207051 NM_015282 NM_001378003 NM_001378004 NM_001378005	NM_001081276 NM_001293300 NM_001293301 NM_029709 NM_177548 NM_001359328 NM_001359329 NM_001359330 NM_001359331 NM_001359334 NM_001359335 NM_001359336 NM_001359337 NM_001359338 NM_001359339 NM_001359340 NM_001359341 NM_001359342 NM_001359343 NM_001359345 NM_001359346 NM_001359347
RefSeq (protein)	NP_001135745 NP_001135746 NP_001193980 NP_056097 NP_001364932 NP_001364933 NP_001364934	NP_001280229 NP_001280230 NP_083985 NP_001346257 NP_001346258 NP_001346259 NP_001346260 NP_001346263 NP_001346264 NP_001346265 NP_001346266 NP_001346267 NP_001346268 NP_001346269 NP_001346270 NP_001346271 NP_001346272 NP_001346274 NP_001346275 NP_001346276
Location (UCSC)	Chr 2: 121.34 – 121.65 Mb	Chr 1: 118.39 – 118.61 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

CLASP1 belongs to a family of microtubule-associated proteins involved in attachment of microtubules to the cell cortex in animals[6] and plants.[7] CLASPs, such as CLASP1, interact with CLIPs (e.g., CLIP1). In animal cells, CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.[5][8] CLASP1 controls the interactions of astral microtubules with the cell cortex in mitosis, which is important for the proper positioning and orientation of the spindle.[9]

References

1. GRCh38: Ensembl release 89: ENSG00000074054 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000064302 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: CLASP1 cytoplasmic linker associated protein 1".
6. Lansbergen G, Grigoriev I, Mimori-Kiyosue Y, et al. (2006). "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta". Developmental Cell. 11 (1): 21–32. doi:10.1016/j.devcel.2006.05.012. PMID 16824950.
7. Ambrose JC, Wasteneys GO (2008). "CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules". Molecular Biology of the Cell. 19 (11): 4730–4737. doi:10.1091/mbc.E08-06-0665. PMC 2575154. PMID 18716054.
8. Maiato H, Fairley EA, Rieder CL, Swedlow JR, Sunkel CE, Earnshaw WC (June 2003). "Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics". Cell. 113 (7): 891–904. doi:10.1016/S0092-8674(03)00465-3. PMID 12837247.
9. Samora, Catarina P.; Mogessie, Binyam; Conway, Leslie; Ross, Jennifer L.; Straube, Anne; McAinsh, Andrew D. (7 August 2011). "MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis". Nature Cell Biology. 13 (9): 1040–1050. doi:10.1038/ncb2297. PMID 21822276. S2CID 8869880.

External links

• Human CLASP1 genome location and CLASP1 gene details page in the UCSC Genome Browser.
• Human MAST1 genome location and MAST1 gene details page in the UCSC Genome Browser.

Further reading

• Ambrose JC, Wasteneys GO (2008). "CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules". Mol Biol Cell. 19 (11): 4730–4737. doi:10.1091/mbc.E08-06-0665. PMC 2575154. PMID 18716054.
• Galjart N (2005). "CLIPs and CLASPs and cellular dynamics". Nat. Rev. Mol. Cell Biol. 6 (6): 487–98. doi:10.1038/nrm1664. PMID 15928712. S2CID 23216754.
• Ishikawa K, Nagase T, Suyama M, et al. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
• Lemos CL, Sampaio P, Maiato H, et al. (2000). "Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization". EMBO J. 19 (14): 3668–82. doi:10.1093/emboj/19.14.3668. PMC 313969. PMID 10899121.
• Akhmanova A, Hoogenraad CC, Drabek K, et al. (2001). "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts". Cell. 104 (6): 923–35. doi:10.1016/S0092-8674(01)00288-4. PMID 11290329.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Maiato H, Fairley EA, Rieder CL, et al. (2003). "Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics". Cell. 113 (7): 891–904. doi:10.1016/S0092-8674(03)00465-3. PMID 12837247.
• Maiato H, Rieder CL, Earnshaw WC, Sunkel CE (2004). "How do kinetochores CLASP dynamic microtubules?". Cell Cycle. 2 (6): 511–4. doi:10.4161/cc.2.6.576. PMID 14504462.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, et al. (2005). "CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex". J. Cell Biol. 168 (1): 141–53. doi:10.1083/jcb.200405094. PMC 2171674. PMID 15631994.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
• Venables JP, Bourgeois CF, Dalgliesh C, et al. (2005). "Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon". Hum. Mol. Genet. 14 (16): 2289–303. doi:10.1093/hmg/ddi233. PMID 16000324.
• Aonuma M, Miyamoto M, Inoue YH, et al. (2006). "Microtubule bundle formation and cell death induced by the human CLASP/Orbit N-terminal fragment". Cell Struct. Funct. 30 (1): 7–13. doi:10.1247/csf.30.7. PMID 16145243.
• Lansbergen G, Grigoriev I, Mimori-Kiyosue Y, et al. (2006). "CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta". Dev. Cell. 11 (1): 21–32. doi:10.1016/j.devcel.2006.05.012. PMID 16824950.
• Mimori-Kiyosue Y, Grigoriev I, Sasaki H, et al. (2006). "Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment". Genes Cells. 11 (8): 845–57. doi:10.1111/j.1365-2443.2006.00990.x. PMID 16866869. S2CID 33744175.
• Pereira AL, Pereira AJ, Maia AR, et al. (2007). "Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore Function". Mol. Biol. Cell. 17 (10): 4526–42. doi:10.1091/mbc.E06-07-0579. PMC 1635371. PMID 16914514.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.