Archaeal H/ACA sRNA

In archaea like in eukaryotes, uridines in various RNAs are converted to pseudouridines by ribonucleoprotein complexes (RNPs) containing H/ACA sRNA.[1][2] Because of their conserved function, these sRNAs are also called small "nucleolar" RNAs (snoRNA) like in eukaryotes, despite no nucleus is present in prokaryotes. By using various computational and experimental approaches in three Pyrococcus genomes seven H/ACA sRNAs and 15 pseudouridine (Ψ) resides on rRNA were identified. One H/ACA motif was shown to guide up to three distinct pseudouridylations.[3] Atypical pseudouridine guide RNA features were identified in Pyrobaculum species. Lack of the conserved 3'-terminal ACA sequence and sometimes lack of 5' portion of the pseudouridylation pocket feature in few conserved Pyrobaculum H/ACA-like sRNAs.[4] A study by Toffano-Nioche et al. proposes an unified structure/function model based on the common structural components in Euryarchaea and Crenarchaea shared by H/ACA and H/ACA-like motifs .[5]

See also

Other archaeal sRNAs:

References
  1. Charpentier, Bruno; Muller, Sébastien; Branlant, Christiane (2005). "Reconstitution of archaeal H/ACA small ribonucleoprotein complexes active in pseudouridylation". Nucleic Acids Research. 33 (10): 3133–3144. doi:10.1093/nar/gki630. ISSN 1362-4962. PMC 1142404. PMID 15933208.
  2. Baker, Daniel L.; Youssef, Osama A.; Chastkofsky, Michael I. R.; Dy, David A.; Terns, Rebecca M.; Terns, Michael P. (2005-05-15). "RNA-guided RNA modification: functional organization of the archaeal H/ACA RNP". Genes & Development. 19 (10): 1238–1248. doi:10.1101/gad.1309605. ISSN 0890-9369. PMC 1132009. PMID 15870259.
  3. Muller, Sébastien; Leclerc, Fabrice; Behm-Ansmant, Isabelle; Fourmann, Jean-Baptiste; Charpentier, Bruno; Branlant, Christiane (May 2008). "Combined in silico and experimental identification of the Pyrococcus abyssi H/ACA sRNAs and their target sites in ribosomal RNAs". Nucleic Acids Research. 36 (8): 2459–2475. doi:10.1093/nar/gkn077. ISSN 1362-4962. PMC 2377435. PMID 18304947.
  4. Bernick, David L.; Dennis, Patrick P.; Höchsmann, Matthias; Lowe, Todd M. (March 2012). "Discovery of Pyrobaculum small RNA families with atypical pseudouridine guide RNA features". RNA. 18 (3): 402–411. doi:10.1261/rna.031385.111. ISSN 1469-9001. PMC 3285929. PMID 22282340.
  5. Toffano-Nioche, Claire; Gautheret, Daniel; Leclerc, Fabrice (2015-09-18). "Revisiting the structure/function relationships of H/ACA(-like) RNAs: a unified model for Euryarchaea and Crenarchaea". Nucleic Acids Research. 43 (16): 7744–7761. doi:10.1093/nar/gkv756. ISSN 1362-4962. PMC 4652768. PMID 26240384.
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