ARHGAP18

Rho GTPase activating protein 18 is a protein that in humans is encoded by the ARHGAP18 gene.[1] The gene is also known as MacGAP and bA307O14.2.[1] ARHGAP18 belongs to a family of Rho GTPase-activating proteins that modulate cell signaling.[2]

Model organisms

Arhgap18 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Micronucleus test	Normal
Heart weight	Normal
Brain histopathology	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of ARHGAP18 function. A conditional knockout mouse line, called Arhgap18^{tm1a(KOMP)Wtsi}[7][8] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][12] Twenty three tests were carried out on mutant mice and one significant abnormality was observed.[5] Fewer than expected homozygous mutant embryos were identified during gestation.[5]

References

1. "Rho GTPase activating protein 18". Retrieved 2011-12-05.
2. Potkin, S. G.; Turner, J. A.; Fallon, J. A.; Lakatos, A.; Keator, D. B.; Guffanti, G.; MacCiardi, F. (2008). "Gene discovery through imaging genetics: Identification of two novel genes associated with schizophrenia". Molecular Psychiatry. 14 (4): 416–428. doi:10.1038/mp.2008.127. PMC 3254586. PMID 19065146.
3. "Salmonella infection data for Arhgap18". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Arhgap18". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Cronin, S.; Tomik, B.; Bradley, D. G.; Slowik, A.; Hardiman, O. (2008). "Screening for replication of genome-wide SNP associations in sporadic ALS". European Journal of Human Genetics. 17 (2): 213–218. doi:10.1038/ejhg.2008.194. PMC 2986065. PMID 18987618.
• Wang, A. G.; Yoon, S. Y.; Oh, J. H.; Jeon, Y. J.; Kim, M.; Kim, J. M.; Byun, S. S.; Yang, J. O.; Kim, J. H.; Kim, D. G.; Yeom, Y. I.; Yoo, H. S.; Kim, Y. S.; Kim, N. S. (2006). "Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags". Biochemical and Biophysical Research Communications. 345 (3): 1022–1032. doi:10.1016/j.bbrc.2006.04.175. PMID 16712791.